Module 12: Cell Cycle/Division Flashcards
(39 cards)
How long is the G1 phase?
8-10 hours
How long is the mammalian cell cycle in culture?
16 hours
How long is S phase?
6-8 hours
How long is the G2 phase?
4-6 hours
How long is the M phase?
30-45 minutes
What does the “G” in the G phases stand for?
Gap
What does FACS stand for?
Fluorescence activated cell sorter
What phases make up interphase?
G1, S, and G2
Flow cytometry measures the ____________ of a population of cells.
DNA content
What is the DNA content during G1 phase?
2n
What is the DNA content during S phase?
2n-4n
What is the DNA content during G2 phase?
4n
What is the DNA content during M phase?
4n
What is mitosis?
Nuclear division
What is cytokinesis?
Cellular division
What are the five stages of M phase?
Prophase, prometaphase, metaphase, anaphase, telophase
What does the “M” in M phase stand for?
“Mitosis”
What is the goal of mitosis?
Ensure sister chromatids are segregated and move to opposite ends of the cell
What allows the cell to package the chromatids for neat segregation?
Chromosome condensation
What allows the cell to stick like chromosomes together?
Cohesin
What are the three main things that occur in prophase?
Chromosome condensation, centrosomes move apart, mitotic spindle starts to form
Which ends of the microtubules anchor to the centrosomes?
The negative ends
What is the open configuration of DNA called?
Euchromatin
What is the close configuration of DNA called?
Heterochromatin