Module 3 (METHODOLOGY)

Question 1

specimens for examination

Answer 1

Bile
Blood
Duodenal contents
Spinal fluid
Sputum
Urine
Vaginal swabs
Biopsy materials
Feces

Question 2

collection of fecal sample

Answer 2

urine & water
must be handled carefully
must be labelled

Question 3

Collection and preservation (fresh specimen)

Answer 3

liquid
soft
formed

Question 4

Collection and preservation
(preservation)

Answer 4

PHYSICAL METHOD
refrigeration

CHEMICAL METHOD
pvA
SCHAUDIN’S FIXATIVE
10% FORMALIN
MIF

Question 5

PROCESSING AND EXAMINATION of fecal sample
(ROUTINE FECALYSIS PROCESSING)

Answer 5

PHYSICAL DESCRIPTION
FORM AND CONSISTENCY
COLOR
ODOR

Question 6

PROCESSING AND EXAMINATION of fecal sample
(ROUTINE FECALYSIS MICROSCOPIC)

Answer 6

DIRECT FECAL SMEAR
NSS
D ANTONI’S SOLUTION
KATO THICK SMEAR

Question 7

PROCESSING AND EXAMINATION of fecal sample
Concentration Method

Answer 7

-FORMALIN-ETHER TECHNIQUE
-ACID-ETHER SEDIMENTATION
-SIMPLE SEDIMENTATION
-MERTHIOLATE-IODINE-FORMALIN CONCENTRATION
-ACID-SODIUM SULFATE

Question 8

PROCESSING AND EXAMINATION of fecal sample
Concentration Method

Answer 8

-SUGAR FLOTATION TECHNIQUE
-WILLIE’S BRINE FLOTATION TECHNIQUE
-LANE’S DIRECT CENTRIFUGAL TECHNIQUE
-MAGNESIUM SULFATE TECHNIQUE
-ZINC SULFATE CENTRIFUGAL FLOTATION TECHNIQUE

Question 9

PROCESSING AND EXAMINATION of fecal sample
Staining Methods

Answer 9

-D’ ANTONI’S
-1% ISOTONIC EOSIN SOLN & 2% BRILLIANT CRESYL BLUE
-AVENSEL VITAL STAIN
-VALET-WEINTEINN OTTO VITAL STAIN
-EOSIN IN SALINE
-BUFFERED METHYLENE BLUE

Question 10

PROCESSING AND EXAMINATION of fecal sample
Staining Methods

Answer 10

-TRICHROME STAINING TECHNIQUE
-MIF FIXATIVE STAIN
-MODIFIED KOHN’S STAIN
-CHLORAZOL BLACK E
-HEIDENHAIN’S IRON HEMATOXYLIN STAIN

Question 11

PROCESSING AND EXAMINATION of fecal sample
(Special Recovery Methods)

Answer 11

CELLULOSE TAPE PREPARATION

EGG COUNT TECHNQUE

Question 12

PROCESSING AND EXAMINATION of fecal sample
(Chemical Examination)

Answer 12

Benzidine Test
Guiac’s Test
Hematest Tablet Test

Question 13

PROCESSING AND EXAMINATION of fecal sample
(Culture Methods)

Answer 13

harada-mori test tube filter paper method

Question 14

PROCESSING AND EXAMINATION of fecal sample
(Immunologic Test)

Answer 14

elisa
rist
immunoblotting

Question 15

PROCESSING AND EXAMINATION of fecal sample
(Others)

Answer 15

animal inoculation test
xenodiagnosis
molecular techniques