Modules 10, 11, 12

Question 1

NLFs that produce H2S

Answer 1

Salmonella and Citrobacter

Question 2

Which tests would differentiate a Salmonella from Citrobacter?

Answer 2

ONPG and Lys Salm = ONPG - , Lys + Citrobacter = ONPG + , Lys -

Question 3

NLF genus that causes Bacillary Dysentery

Answer 3

Shigella

Question 4

Two types of infections causes by Salmonella organisms

Answer 4

Enteric fever and Gastroenteritis

Question 5

Why would Citrobacter be worked up as a possible Salmonella?

Answer 5

NLF at 24 hours, H2S positive

Question 6

A lactose negative organism (genus) that produces H2S, is deaminase negative and lysine positive

Answer 6

Salmonella

Question 7

Serotype of Salmonella that is ornithine negative and citrate negative

Answer 7

Salmonella typhi

Question 8

Shigella dysenteriae group:

Answer 8

A

Question 9

Shigella flexneri group:

Answer 9

B

Question 10

Shigella boydii group:

Answer 10

C

Question 11

Shigella sonnei group:

Answer 11

D

Question 12

Group A Shigella

Answer 12

Shigella dysenteriae

Question 13

Group B Shigella

Answer 13

Shigella flexneri

Question 14

Group C Shigella

Answer 14

Shigella boydii

Question 15

Group D Shigella

Answer 15

Shigella sonnei

Question 16

Species of Shigella that is ONPG positive and Ornithine positive

Answer 16

Shigella sonnei

Question 17

Common characteristics of the family Enterobacteriaceae

Answer 17

Gram negative rods, non-spore forming
Facultative anaerobes with best growth in oxygen
Oxidase negative
Glucose fermeters
Reduces nitrate to nitrite
Catalase positive (most)
Growth on MacConkey agar

Question 18

The reaction in the butt of a TSI tube for Enterobacteriaceae

Answer 18

Acid (yellow); all Enterobacteriaceae ferment glucose

Question 19

Top 3 Enterobacteriaceae commonly isolated from clinical material

Answer 19

Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis

Question 20

An organism that forms dark pink dry colonies on MAC and is spot indole positive from colonies on BAP is most likely:

Answer 20

E. coli

Question 21

Species of Enterobacter most often isolated from clinical material

Answer 21

Enterobacter cloacae

Question 22

Two species of Klebsiella commonly isolated from clinical material

Answer 22

Klebsiella pneumoniae, Klebsiella oxytoca

Question 23

Test to differentiate K. pneumoniae from K. oxytoca

Answer 23

Indole: K. pneumoniae indole neg, K. oxytoca indole pos

Question 24

Genus that is almost always resistant to Ampicillin, Carbenicillin, and Ticarcillin

Answer 24

Klebsiella

Question 25

Antimicrobial treatment for Klebsiella

Answer 25

Aminoglycosides (Gentamicin, Kanamycin, Tobramycin, Amikacin) and some Cephalosporins

Question 26

Two species of Shigella most often isolated from clinical material

Answer 26

Shigella flexneri and Shigella sonnei

Question 27

Most common species of Citrobacter

Answer 27

Citrobacter freundii