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Flashcards in Molecular Biology Deck (19)
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1

What is molecular biology?

The study of the relationships between structures and function of biological molecules

2

What is bacterial DNA replication called?

semi-conservative

3

Describe bacterial transcription and translation?

RNA attaches to promoter on DNA, synthesis MRNA until termination, but ribosomes attach as no nuclear membrane and translate before termination

4

What is a codon?

three bases of DNA which codes for an amino acids

5

Why is DNA degenerate?

Some combinations of codons code for the same amino acid

6

What does the polymerase chain reaction do?

Reproduces sections of DNA rapidly

7

What is required for PCR?

DNA, polymerase, buffer, primers

8

What are the three phases of PCR?

Denature- separate strands, anneal- primers, extend- replicate

9

What temperature separates the two DNA strands?

90-100 degrees

10

What temperature do the primers attach?

30-65 degrees

11

What temperature are new DNA stands synthesised at

60-70 degrees

12

What are the applications of PCR?

Taxonomy, Diagnostics, Cloning

13

What are the two types of old DNA sequencing?

Maxam and Gilbert
Sanger

14

What does maxam and Gilbert sequencing work?

Enzyme cleavage, developed on x-ray film

15

What are the new types of sequencing?

Illumina sequencing

16

What is phylogenic analysis?

Analysation of ribosomal RNA, MLST, whole genome sequence

17

What is MLST?

Multi-locus sequence typing

18

What does MLST use?

Uses 6-8 housekeeping genes and PCR to DNA sequence

19

What does whole genome sequence analysis allow use for?

Monitoring transmission, enables vaccine design