Flashcards in Molecular Biology Deck (19)
What is molecular biology?
The study of the relationships between structures and function of biological molecules
What is bacterial DNA replication called?
Describe bacterial transcription and translation?
RNA attaches to promoter on DNA, synthesis MRNA until termination, but ribosomes attach as no nuclear membrane and translate before termination
What is a codon?
three bases of DNA which codes for an amino acids
Why is DNA degenerate?
Some combinations of codons code for the same amino acid
What does the polymerase chain reaction do?
Reproduces sections of DNA rapidly
What is required for PCR?
DNA, polymerase, buffer, primers
What are the three phases of PCR?
Denature- separate strands, anneal- primers, extend- replicate
What temperature separates the two DNA strands?
What temperature do the primers attach?
What temperature are new DNA stands synthesised at
What are the applications of PCR?
Taxonomy, Diagnostics, Cloning
What are the two types of old DNA sequencing?
Maxam and Gilbert
What does maxam and Gilbert sequencing work?
Enzyme cleavage, developed on x-ray film
What are the new types of sequencing?
What is phylogenic analysis?
Analysation of ribosomal RNA, MLST, whole genome sequence
What is MLST?
Multi-locus sequence typing
What does MLST use?
Uses 6-8 housekeeping genes and PCR to DNA sequence