Molecular transcription Flashcards
(21 cards)
4 characteristics of genetic material
replication
expression
storage
variation
Unambiguous
one codon per amino acid
Degenerate
several amino acids associate its several codons
Non-overlapping
Each base appears in only one triplet- each base is only read once
True or false: codons are uninterrupted in mRNA, after introns are removed
true
what is deamination?
removal of an amino group. The base pair cytosine changes to uracil
True or false: ciliates scramble their germline genome
True
True or false: overlapping genes share RNA space, not DNA
False. they share DNA space
Is transcription and translation sequential or simultaneous in eukaryotes and prokaryotes?
Eukaryotes are sequential, prokaryotes are simultaneous
One possible problem following transcription is compartmentalizations, what is that?
When the newly synthesized mRNA strand remains in the nucleus vs. going into the cytoplasm
Which rna polymerase is used during transcription?
2
Which 3 transcription factors are involved in TF2D?
Tbp
Inr
Dpe
What comes after TF2D? And what one factor helps it out?
TF2B
BRE
TF2 E attracts which other factor
TF2 H
TF2F recruits which other factors
TF2E and TF2H
TF 2 H contains which helicase. Hint: think of xeroderma pigmentosum
XPB
What happens when TF2H is phosphorylated
All other TF2 factors disassociate allowing rna pol 2 to change shape and being transcribing
Which part of the dna does the activator protein bind to?
The enhancer
What protein cause a pause in transcription?
NELF
How does topoisomerase help during transcription?
Relieves the superhelical stress
True or false: rna polymerases have proofreading sites
False
