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HPCT > MOUNTING/LABELING > Flashcards

MOUNTING/LABELING Flashcards

1
Q

last step on the tissue processing

A

Mounting

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2
Q

syrupy fluid applied between the section and the coverslip IMMEDIATELY after staining

A

Mounting medium

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3
Q

Mounting a tissue specimen is essential for preserving the specimen during ______ (for long periods of time) as well as for enhancing _____ quality during microscopy

A

storage

image

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4
Q

Histopath slides → can last up to _____ years retention period

A

10

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5
Q

Mounting medium:

[Action] tissue section. Not ____ or blurred

A

flatten

wavy
blurred

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6
Q

If an unmounted stained section is seen in the
microscope, differences in ______ between the glass slides and the tissue components, may lead to difference in _________; hence, very little microscopic detail can usually be appreciated.
◆ Some parts of tissue sections are [2]

A

light refraction

length dispersion

blurry/wavy

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7
Q

We need to use _______ to secure properly the glass slide and to prevent movement and washing out or scratches

A

mounting medium

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8
Q

Mounting medium flatten out sections for what purpose?

A

fine details of cells

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9
Q

Mounting medium purposes [4]

A

promote spx adhesion
promote glass slide adhesion
prevent spx movement
spx preserve

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10
Q

Characteristics of a Good Mounting medium

A
  1. freely miscible w/ xylene/toluene
  2. should not dry quickly
  3. should not dissolve/fade out tissue sections
  4. should not crack or produce artefactual granularity on the slide upon drying
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11
Q

Dryness will cause _____ on glass slides, resulting to the tendency of tissue section to be destroyed.

A

cracks

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12
Q

artefactual granularity causes ?

A

bubbles

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13
Q

No bubbles or cracks must be produced
because you will not appreciate the cells’ [2]

A

nucleus

cytoplasm

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14
Q

Ratio of speed of light in air and in a specific medium

A

Refractive Index

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15
Q

Value to which light can pass through a
medium

A

RI

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16
Q

Refractive index should be as near as possible to
that of the glass which is [#]

A

1.518

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17
Q

As much as possible the RI must be higher
than the RI of ____ to consider that the mounting medium is of good quality

A

glass

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18
Q

________
They are designed to mount water miscible preparations directly from water where the stained is removed or decolorized with ____ or _____.

Because there are components of tissue slides that are dissolved when using alcohol or xylene: ______

A

Aqueous Media

alcohol
xylene

lipids

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19
Q

Aqueous Media is made up of? [4] [+function]

A

Gelatin, Glycerin Jelly or Gum Arabic- solidifigcation

Glycerol- prevent cracking/drying

Sugar- increase RI

Merthiolate- preservative

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20
Q

Aqueous Media examples [6]

A

Water
Mineral Oil

Glycerin
Levulose

Brun’s Fluid
Farrant’s medium

Von Apathy’s Gum Syrup
Karo Corn Syrup

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21
Q

Has low RI
➔ Moderately transparent
➔ Evaporates easily

A

Water

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22
Q

➔ Good only for temporary mounting

A

Water

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23
Q

May be used also as preservative for Aqueous Media other than merthiolate

A

Glycerin

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24
Q

➔ Has high refractive index, RI 1.46
➔ Last for a few minutes

A

Glycerin

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25
Q

Glycerin Jelly RI:

A

1.47

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26
Q

Farrant’s medium RI:

A

1.43

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27
Q

Useful temporary mounting media for Romanowsky stained smear

A

Mineral Oil

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28
Q

Von Apathy’s Gum Syrup RI:

A

1.52

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29
Q

Von Apathy’s Gum Syrup is made up of? [5]

A

Distilled water
Sucrose

Pure gum arabic
Pure cane sugar

Thymol

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30
Q

Levulose/Fructose RI

A

1.5

31
Q

Brun’s Fluid is made up of? [4]

A

Distilled water
Glucose
Glyrcerin

Spirit of Camphor

32
Q

For preparations that have been dehydrated and cleared with xylene or toluene

A

Resinous media

33
Q

Recommended for majority of staining methods

A

Resinous media

34
Q

Resinoud media is divided by [2]

A

Natural type of resins

Synthetic type of resins

35
Q

Canada Balsam
Natural resin extracted from the ______ Tree → ________.

A

Canadian

Abus Balmasea

36
Q

type of resin that is transparent/colorless

A

Canada Balsam

37
Q

type of resin that sets to a hard consistency when dry

A

Canada Balsam

38
Q

There are two categories of mounting media

A

Organic/Aqueous
Hydrophobic/Hydrophilic

39
Q

________can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue

Examples:

A

Organic mounting media

Diaminobenzidine
DAB

40
Q

Aqueous mounting media is generally suitable for all _______ label/______ combinations and _____ labels.

A

enzymatic labels

chromogen combinations

fluorescent labels

41
Q

Synthetic Brands Superior than Canada Balsam [5]

A
  1. DPX of Kirkpatrick & Lendrum
  2. Clarite X
  3. Permount, Clearmount, Histosec, Harleco synthetic resin, Entallan
  4. XAM
  5. Eukitt
42
Q

meaning of DPX

A

Dibutyl Phthalate & Xyelene

43
Q

DPX of Kirkpatrick & Lendrum is also called as

A

Resin “distrene 80”

Tricresyl phosphate/ Dibutyl phthalate

44
Q

a good mounting medium but shrink somewhat on drying

A

DPX of Kirkpatrick & Lendrum

45
Q

DPX of Kirkpatrick & Lendrum RI:

A

1.52

46
Q

When mounting medium has ____, you can see
in the ____ of glass slides, the mounting medium might shrink out

A

dried

edges

47
Q

One of the widely used mounting media

A

Clarite X

48
Q

Clarite X RI:

A

1.544

49
Q

Generally preferred as compared to DPX

A

Clarite X

50
Q

XAM RI:

A

1.52

51
Q

Synthetic resin mix in xylene, available in [color] (disadvantage) or [color] solution

A

XAM

pale yellow
colorless

52
Q

Dries quickly without retraction, and preserves the. stains well

A

XAM

53
Q

It is an adhesive and specimen preservative

A

Eukitt

54
Q

Eukitt contains [2]

A

5% Acrylic resin
55% Xylene

55
Q

In lab we used _____ to mount the slides

A

Eukitt

56
Q

Eukitt Properties:
◆ ______ drying with minimal ______.
◆ RI close to the glass (______)
◆ ______ neutral, little sensitivity to _____
◆ No reaction to _____. (Does not change
_____/_____)
◆ Non-______, unaffected by [hot/cold] and ____.
◆ Stays optically clear for over _____ years
◆ Does not _____.

A

Fast
air bubbles

1.510

chemically
water

staining
color/pH

yellowing
cold
UV

10

crystallize

57
Q

Finished slides easily reworked by immersing previously mounted slides in ______ to remove coverslip.

A

xylene

58
Q

In histopath if you have to remove coverslip you have to dip in _____ or _______ that is miscible to mounting

A

saline

clearing agent

59
Q

The slides in mounting media may then be incubated at ___*C for _____ hrs. After mounting, to harden the medium.

A

37C

12-24 hrs [half-whole day]

60
Q

Setting may be hastened in a ______ at 50*C for ___ hrs.

A

hot oven

2 hrs

61
Q

After _______ staining, mounting media are used to adhere a coverslip to a tissue section or cell smear.

A

immunochemical staining

62
Q

Mounting media also play an important role in
improving the _____ and ____ of an image during microscopy.

A

clarity
contrast

63
Q

Sealing the margin of the coverslip

A

Ringing

64
Q

Aside from putting mounting medium you can _____
media to seal the glass slide and cover slip

A

pit

65
Q

a ringing agent and is made of [2 parts]

A

Krognig cements

paraffin wax
4-9 parts of powdered colophonium resin

66
Q

The stained section on the slide is covered with a thin piece of glass to protect the tissue from scratches/ damages, to provide better optical quality for viewing under the microscope, and to
preserve the tissue section for years to come.

A

Coverslipping

67
Q

With used of coverslip it can preserve the _____
ot tissue it can protect from scratches and to preserve long period of time for years cover slip is used to preserve the section

A

integrity

68
Q

Cover slip are identified by ______ which pertain to the thickness

A

number

69
Q

Thickness and transparency of the cover slip is [directly/inversely proportional]

As thickness increases, transparency is [increased/reduced].

A

inversely proportional

reduced

70
Q

Cover glasses come in different sizes, namely:
[4]

A

18x18mm
20x20mm
22x22mm
25x25mm

71
Q

The choice of cover glass depends on the ____ of the tissue to be mounted.

A

size

72
Q

Slide with frosted edge, use ________to label the specimen

Slide with non-frosted edge, use ________to label the specimen

A

LED pencil

Diamond pencil

73
Q

The labels may be [3]
on each

A

written
etched
embossed

74
Q
A

HPCT (5 decks)

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