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HPCT > MOUNTING/LABELING > Flashcards

MOUNTING/LABELING Flashcards

(74 cards)

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1
Q

last step on the tissue processing

A

Mounting

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2
Q

syrupy fluid applied between the section and the coverslip IMMEDIATELY after staining

A

Mounting medium

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3
Q

Mounting a tissue specimen is essential for preserving the specimen during ______ (for long periods of time) as well as for enhancing _____ quality during microscopy

A

storage

image

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4
Q

Histopath slides → can last up to _____ years retention period

A

10

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5
Q

Mounting medium:

[Action] tissue section. Not ____ or blurred

A

flatten

wavy
blurred

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6
Q

If an unmounted stained section is seen in the
microscope, differences in ______ between the glass slides and the tissue components, may lead to difference in _________; hence, very little microscopic detail can usually be appreciated.
◆ Some parts of tissue sections are [2]

A

light refraction

length dispersion

blurry/wavy

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7
Q

We need to use _______ to secure properly the glass slide and to prevent movement and washing out or scratches

A

mounting medium

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8
Q

Mounting medium flatten out sections for what purpose?

A

fine details of cells

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9
Q

Mounting medium purposes [4]

A

promote spx adhesion
promote glass slide adhesion
prevent spx movement
spx preserve

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10
Q

Characteristics of a Good Mounting medium

A
  1. freely miscible w/ xylene/toluene
  2. should not dry quickly
  3. should not dissolve/fade out tissue sections
  4. should not crack or produce artefactual granularity on the slide upon drying
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11
Q

Dryness will cause _____ on glass slides, resulting to the tendency of tissue section to be destroyed.

A

cracks

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12
Q

artefactual granularity causes ?

A

bubbles

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13
Q

No bubbles or cracks must be produced
because you will not appreciate the cells’ [2]

A

nucleus

cytoplasm

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14
Q

Ratio of speed of light in air and in a specific medium

A

Refractive Index

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15
Q

Value to which light can pass through a
medium

A

RI

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16
Q

Refractive index should be as near as possible to
that of the glass which is [#]

A

1.518

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17
Q

As much as possible the RI must be higher
than the RI of ____ to consider that the mounting medium is of good quality

A

glass

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18
Q

________
They are designed to mount water miscible preparations directly from water where the stained is removed or decolorized with ____ or _____.

Because there are components of tissue slides that are dissolved when using alcohol or xylene: ______

A

Aqueous Media

alcohol
xylene

lipids

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19
Q

Aqueous Media is made up of? [4] [+function]

A

Gelatin, Glycerin Jelly or Gum Arabic- solidifigcation

Glycerol- prevent cracking/drying

Sugar- increase RI

Merthiolate- preservative

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20
Q

Aqueous Media examples [6]

A

Water
Mineral Oil

Glycerin
Levulose

Brun’s Fluid
Farrant’s medium

Von Apathy’s Gum Syrup
Karo Corn Syrup

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21
Q

Has low RI
➔ Moderately transparent
➔ Evaporates easily

A

Water

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22
Q

➔ Good only for temporary mounting

A

Water

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23
Q

May be used also as preservative for Aqueous Media other than merthiolate

A

Glycerin

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24
Q

➔ Has high refractive index, RI 1.46
➔ Last for a few minutes

A

Glycerin

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25
Glycerin Jelly RI:
1.47
26
Farrant's medium RI:
1.43
27
Useful temporary mounting media for Romanowsky stained smear
Mineral Oil
28
Von Apathy's Gum Syrup RI:
1.52
29
Von Apathy's Gum Syrup is made up of? [5]
Distilled water Sucrose Pure gum arabic Pure cane sugar Thymol
30
Levulose/Fructose RI
1.5
31
Brun's Fluid is made up of? [4]
Distilled water Glucose Glyrcerin Spirit of Camphor
32
For preparations that have been dehydrated and cleared with xylene or toluene
Resinous media
33
Recommended for majority of staining methods
Resinous media
34
Resinoud media is divided by [2]
Natural type of resins Synthetic type of resins
35
Canada Balsam Natural resin extracted from the ______ Tree → ________.
Canadian Abus Balmasea
36
type of resin that is transparent/colorless
Canada Balsam
37
type of resin that sets to a hard consistency when dry
Canada Balsam
38
There are two categories of mounting media
Organic/Aqueous Hydrophobic/Hydrophilic
39
________can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue Examples:
Organic mounting media Diaminobenzidine DAB
40
Aqueous mounting media is generally suitable for all _______ label/______ combinations and _____ labels.
enzymatic labels chromogen combinations fluorescent labels
41
Synthetic Brands Superior than Canada Balsam [5]
1. DPX of Kirkpatrick & Lendrum 2. Clarite X 3. Permount, Clearmount, Histosec, Harleco synthetic resin, Entallan 4. XAM 5. Eukitt
42
meaning of DPX
Dibutyl Phthalate & Xyelene
43
DPX of Kirkpatrick & Lendrum is also called as
Resin "distrene 80" Tricresyl phosphate/ Dibutyl phthalate
44
a good mounting medium but shrink somewhat on drying
DPX of Kirkpatrick & Lendrum
45
DPX of Kirkpatrick & Lendrum RI:
1.52
46
When mounting medium has ____, you can see in the ____ of glass slides, the mounting medium might shrink out
dried edges
47
One of the widely used mounting media
Clarite X
48
Clarite X RI:
1.544
49
Generally preferred as compared to DPX
Clarite X
50
XAM RI:
1.52
51
Synthetic resin mix in xylene, available in [color] (disadvantage) or [color] solution
XAM pale yellow colorless
52
Dries quickly without retraction, and preserves the. stains well
XAM
53
It is an adhesive and specimen preservative
Eukitt
54
Eukitt contains [2]
5% Acrylic resin 55% Xylene
55
In lab we used _____ to mount the slides
Eukitt
56
Eukitt Properties: ◆ ______ drying with minimal ______. ◆ RI close to the glass (______) ◆ ______ neutral, little sensitivity to _____ ◆ No reaction to _____. (Does not change _____/_____) ◆ Non-______, unaffected by [hot/cold] and ____. ◆ Stays optically clear for over _____ years ◆ Does not _____.
Fast air bubbles 1.510 chemically water staining color/pH yellowing cold UV 10 crystallize
57
Finished slides easily reworked by immersing previously mounted slides in ______ to remove coverslip.
xylene
58
In histopath if you have to remove coverslip you have to dip in _____ or _______ that is miscible to mounting
saline clearing agent
59
The slides in mounting media may then be incubated at ___*C for _____ hrs. After mounting, to harden the medium.
37C 12-24 hrs [half-whole day]
60
Setting may be hastened in a ______ at 50*C for ___ hrs.
hot oven 2 hrs
61
After _______ staining, mounting media are used to adhere a coverslip to a tissue section or cell smear.
immunochemical staining
62
Mounting media also play an important role in improving the _____ and ____ of an image during microscopy.
clarity contrast
63
Sealing the margin of the coverslip
Ringing
64
Aside from putting mounting medium you can _____ media to seal the glass slide and cover slip
pit
65
a ringing agent and is made of [2 parts]
Krognig cements paraffin wax 4-9 parts of powdered colophonium resin
66
The stained section on the slide is covered with a thin piece of glass to protect the tissue from scratches/ damages, to provide better optical quality for viewing under the microscope, and to preserve the tissue section for years to come.
Coverslipping
67
With used of coverslip it can preserve the _____ ot tissue it can protect from scratches and to preserve long period of time for years cover slip is used to preserve the section
integrity
68
Cover slip are identified by ______ which pertain to the thickness
number
69
Thickness and transparency of the cover slip is [directly/inversely proportional] As thickness increases, transparency is [increased/reduced].
inversely proportional reduced
70
Cover glasses come in different sizes, namely: [4]
18x18mm 20x20mm 22x22mm 25x25mm
71
The choice of cover glass depends on the ____ of the tissue to be mounted.
size
72
Slide with frosted edge, use ________to label the specimen Slide with non-frosted edge, use ________to label the specimen
LED pencil Diamond pencil
73
The labels may be [3] on each
written etched embossed
74

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