MR CELL viability Flashcards
(8 cards)
Which cell viability assay is inappropriate to use when measuring apoptosis 30 minutes after adding a apoptosis-inducing drug to a cancer cell line?
a.
Annexin V
b.
ATP
c.
LDH release
d.
MTS
e.
Caspase activation
C. LDH RELEASE
Which cell viability assay/stain is most appropriate to use in a high-throughput experiment to screen a panel of drugs for toxicity
a.
ATP
a. ATP
b. Annexin V
c. 7-AAD
Trypan blue
d. Trypan blue
Erythrosine B
ATP
Which fixative should be used in an experiment to measure phosphorylated transcription factors using fluorophore-labelled antibodies?
a. Triton X-100
b. SDS
c. Glutaraldehyde
d. Methanol
e.Formaldehyde
Methanol
Which membrane dye exclusion reagent is most appropriate to use in an experiment where samples need to be fixed during staining?
a. MitoTracker
b. 7AAD
c. Propidium iodide
d. Amine-reactive dyes
e.Trypan blue
Amine reactive dyes
Which of the following cell viability assays doesn’t not rely on enzymatic activity?
a. Resazurin
b. WST-1
c. Trypan blue
d. LDH release
e.Calcein AM
Trypan blue
Which of the following is NOT a goal of fixation and permeabilization?
a. To ensure tissue structure is maintained after sampling.
b. To maintain the spatial arrangement of cellular molecules.
c. To maintain cells in a viable, metabolically active state.
d. To enable access to intra-cellular components.
e.
To maintain accurate cell morphology
To maintain cells in a viable, metabolically active state
Which of the following is NOT a permeabilization agent?
a. NP-40
b. Triton X-100
c. Methanol
d. Saponin
e.
Formaldehyde
Formaldehyde
Which permeabilization method should be used in an experiment to measure the expression of a protein expressed on the endoplasmic reticulum using fluorophore-labelled antibodies?
a. SDS
b.Formaldehyde
C. Methanol
D.Saponin
E.Triton X 100
Saponin
