MYCOLOGY LAB ID METHODS

Question 1

what is the gold standard of mycology id methods

Answer 1

culture

Question 2

what newer diagnostic testing is mostly used for yeast

Answer 2

MALDI-TOF

Question 3

what newer diagnostic testing is mostly used for bacteria

Answer 3

micro scan, vitex, and phoenix

Question 4

what kind of lab uses gram stain, modified acid fast stain, wright stain, and giemsa stain

Answer 4

clinical lab

Question 5

what kind of lab uses periodic acid-schiff, H & E, Aomori’s methenamine silver

Answer 5

pathology lab

Question 6

T. or F: calcofluor white is used for fluorescence microscopy

Answer 6

T

Question 7

what makes up the KOH wet mount

Answer 7

10% potassium hydroxide and specimen

Question 8

what is the purpose of the KOH wet mount

Answer 8

dissolve away the non fungal elements

Question 9

is a KOH met mount stained

Answer 9

no

Question 10

T or F: KOH wet mount can be examined hours after it is made

Answer 10

F

Question 11

what is the purpose of India ink

Answer 11

to ID encapsulated yeast

Question 12

what yeast does India ink ID

Answer 12

cryptococcus

Question 13

what kind of specimen does India ink use

Answer 13

CSF

Question 14

what stain is used to ID fungal growth on agar

Answer 14

lactophenol cotton blue

Question 15

lactophenol cotton blue: what is the purpose of phenol

Answer 15

kills organisms

Question 16

lactophenol cotton blue: what is the purpose of lactic acid

Answer 16

preserves fungal structures

Question 17

lactophenol cotton blue: what is the purpose of cotton blue

Answer 17

stains the chitin in the cell wall

Question 18

what are the 2 methods used to pick up fungus off agar

Answer 18

tape or tease mount

Question 19

what does calcofluor white bind to

Answer 19

polysaccharides of chitin in fungal cell wall

Question 20

T or F: calcofluor white works on any fungus

Answer 20

F

Question 21

what kind of morphology can be examined from culture

Answer 21

micro and macroscopic

Question 22

what is the primary inoculation of culture

Answer 22

specimen on the agar plate

Question 23

what is the secondary. isolation of culture

Answer 23

put colonies onto a new type of media

Question 24

what kind of organism does chromogenic agar ID

Answer 24

yeast

Question 25

what kind of organism does cornmeal agar ID

Answer 25

C. albicans chlamydospores

Question 26

what kind of organism does bird seed agar ID

Answer 26

cryptococcus

Question 27

what does some media have to prevent overgrowth of bacteria from mixed cultures

Answer 27

antibacterial ingredients

Question 28

what do microscans ID

Answer 28

yeast

Question 29

what does microscan use to ID organisms

Answer 29

substrate, carbohydrate, and enzyme differentiation

Question 30

what is the principle of DNA probes

Answer 30

nucleic acid hybridization

Question 31

how do you know if there are ab present in immunodiffusion testing

Answer 31

a line of precipitation appears

Question 32

is immunodiffusion qualitative or quantitative

Answer 32

qualitative

Question 33

in complement fixation testing is the is no color change what does that mean

Answer 33

there are fungal Ab in pt serum --> complement does not lyse sheep blood --> pos

Question 34

in complement fixation testing is the is a color change what does that mean

Answer 34

there are not fungal Ab in pt serum --> complement does lyse sheep blood --> neg

Question 35

what is the purpose of antifungal susceptibility testing

Answer 35

produce MIC for pt therapy, inform epidemiological studies, and track drug resistance

Question 36

when will a doc request antifungal susceptibility testing

Answer 36

invasive fungal infection, if drug resistance is suspected, or pt theory is not working

Question 37

if there is no growth around an antibiotic the organism is ______

Answer 37

sensitive to that antibiotic

Question 38

if there is growth around an antibiotic the organism is ______

Answer 38

resistant to that antibiotic

Question 39

if a fungus does have some zone of no growth but there is a little what is that called

Answer 39

trailing growth

Question 40

what organism does triazole treat

Answer 40

aspergillus