nanooptamology Flashcards
(16 cards)
around ___ corneal graft transplants are done each year in the US
__% end in rejection, mostly due to __ and __
40,000
10%
medicine compliance & immunologic graft rejection
around ___ corneal graft transplants are done each year in the US
__% end in rejection, mostly due to __ and __
40,000
10%
medicine compliance & immunologic graft rejection
___ is the transparent part of the eye that covers the iris & pupil
cornea
role of the cornea is to ___, accounting for ~ ___ of the eye’s total optical power
refract light
2/3
if the corneal stroma develops visually significant opacity, irregularity, or edema, a cornea of a deceased donor can be ___
transplanted
what is the drug treatment in the cornea transplant in the rats
PLGA NP
this drug, ___, was the ___ used to treat graft recipients
DSP
immunosuppressive corticosteroid
in the rat study, ___ rats were used as corneal donors and ___ rats were used as the acceptors
fisher
lewis
in the rat study, after the PK surgery, the recipient rats were randomly divided into 4 groups and treated with variant 50 mL SC injection
- 4 rats: saline injection
- 5 rats: placebo NP
- 5 rats: free DSP aq (2 mg/mL)
- 6 rats: DSP-NP (2 mg DSP/mL)
why were rats used in the cornea transplant study over mice?
larger eyes
in the rat study, failure of graft was determined using a specific set of grading parameters based on ___, ___, and ___
corneal transparency
edema
neovascularization
(scale of 0-4) 0= good
an increase in opacity, edema, or neovascularization all ___
possible graft rejection
an increase in opacity, edema, or neovascularization all ___
possible graft rejection
describe results of the 4 diff rat groups
groups 1-3 all rejected the graft
corneal histological exam after the rat study- the 4 diff group results
the cornea was massively thickened in the control groups (1 & 2), focally thickened when treated with free drug (3) and normal appearing when treated with DSP-NP
to measure in vivo biodistribution, DSP was labeled with titrium-H3 and delivered via SC injection by either free DSP or NP-DSP
the conjunctival injection site, aqueous humor, vitreous humor, and blood were all measured for DSP presence at time points
- up to 3 days, found DSP in vitreous and aqueous humor
