Nomenclature

Question 1

How to indicate substrains?

Answer 1

/

Question 2

Describe the appearance of a lab code and it’s location.

Answer 2

1-5 letters, starting with an uppercase and followed by lowercase. Follows ‘/’. May present in cumulative strings

Question 3

How to list a F1 hybrid

Answer 3

Female ‘x’ Male

Question 4

How to list a RI strain from two inbred parental strains

Answer 4

Female’X’Male - Specific line

Question 5

How to list a mutant gene

Answer 5

Parent gene (italics, starting with uppercase letter), mutant allele in superscript

Question 6

How would a co-isogenic gene look?

Answer 6

Strain/Labcode-Gene(italics), allele(superscript)

Question 7

How to list a transgene

Answer 7

Strain - Tg(brief description)#(assigned)Labcode
Example: FVB-Tg(MMTV-Erb2)1Led
No part is ever italicized - Not part of native genome

Question 8

How to list transgene that causes insertional mutation in identified endogenous gene?

Answer 8

Gene (italics), tg1Labcode (superscript)

Question 9

How to list targeted mutation.

Answer 9

Recipient;donor-Gene (italics), tm#Labcode (superscript)

Question 10

How to list gene replacement or knock in

Answer 10

Gene replaced, gene added (superscript)

Question 11

At which generation is backcrossing complete for a congenic line? How is this noted?

Answer 11

N10, noted with a ‘.’ between background and donor strain

Question 12

What occurs after N5 backcrossing?

Answer 12

Incipient congenic, ‘;’ between background an donor strain

Question 13

How is the background strain indicated if of mixed genetic origin?

Answer 13

STOCK

Question 14

How is the donor strain indicated if of mixed origin?

Answer 14

Cg

Question 15

How are outbred stock that meet specific criteria designated?

Answer 15

Labcode:Stock

Question 16

How are animal and human genes indicated?

Answer 16

All alleles. Human genes all capitalized, animal genes only have the first letter capitalized.

Question 17

How are alleles written?

Answer 17

Italics. Capitalized in dominant inheritance, lowercase if recessive inheritance.

Question 18

How are proteins indicated?

Answer 18

No italics, all capitalized.

Question 19

How is a stock indicated?

Answer 19

Lab Code: Stock (Origin of stock)

Question 20

How many brother x sister matings constitute a strain? Why?

Answer 20

20 brother-sister or parent-offspring. After this point uniform genome with only 0.01% residual heterozygosity.

Question 21

How is a strain indicated?

Answer 21

Unique, brief symbol with uppercase letters and numbers. Related inbred strain names should indicate relatedness.

Question 22

How are substrains indicated?

Answer 22

Strain/Substrain

Question 23

Describe a Laboratory Registration Code? Who creates these?

Answer 23

1-5 letters. Assigned by Institute of Lab Animal Research (ILAR), now Board of Animal Health Science, Conservation, and Research (BAHSCR)

Question 24

If a lab creates multiple substrains, how are those indicated?

Answer 24

Strain/SerialNumberSubstrain
FL/1Re, FL/2Re

Question 25

What are exceptions to naming of substrains?

Answer 25

BALB/c - Not a substrain DBA/1 and DBA/2 - Separate strains, not substrains

Question 26

How are substrains of substrains developed? How are they indicated?

Answer 26

Arise when colony is maintained by a new investigator, or a new colony is established. Strain/SubstrainSubsubstrain

Question 27

How are F1 hybrids indicated? F2?

Answer 27

(Maternal x Paternal) F#/LabCode. Shortened to MPF#/LabCode (B6D2F1/J). F2 hybrids and beyond are indicated in the same way.

Question 28

How are recombinant inbred strains indicated?

Answer 28

Think railroad crossing! Maternal X Paternal Serial# No punctuation, unless paternal strain ends in number. If so, hyphen then serial #

Question 29

When is the generational term 'N' used?

Answer 29

Backcrossing. F is for inbreeding.

Question 30

How are congenic mice indicated?

Answer 30

Recipient.Donor-Gene^allele/Lab code

Question 31

What is an incipient congenic? How is this indicated?

Answer 31

Not backcrossed for 10 full generations. Indicated by ;

Question 32

Hi!

Answer 32

Doing great!

Question 33

How are coisogenic mice indicated?

Answer 33

Strain/Substrain - Gene^Allele

Question 34

How is an endonuclease mediated transgenic mouse indicated?

Answer 34

Strain-Gene^em#LabCode

Question 35

How is a consomic strain described?

Answer 35

Host strain-Chromsome^Donor

Question 36

How is the rearing or microbiological status of stocks indicated?

Answer 36

Lab code:StockRearing or microstatus

Question 37

How is the origin or remove ancestry of a stock indicated?

Answer 37

Lab code: Stock (Stock2)

Question 38

How are mutant stocks indicated? What if they are heterozygous for the gene of interest?

Answer 38

Lab Code: Stock - Gene If het: Lab code: Stock - +Gene

Question 39

What is a recombinant congenic strain? How is it indicated?

Answer 39

Crossing two inbred strains followed by a few backcrosses. Recipient c donor

Question 40

What are advanced intercross lines. How are they indicated?

Answer 40

Producing F2 of two inbred strains and then avoiding sibling mating to increase possibility of tightly linked genes recombining. Lab code: Inbred strain 1, inbred strain 2 G G is generation number, starts after F2 generation.

Question 41

What is a conplastic strain? How is it indicated?

Answer 41

Cytoplasmic genome (mitochondria) is put on a different strain that maintains its nuclear genome. Nuclear genome strain -mt^Cytoplasmic gene donor

Question 42

How are segregating inbred mutant strains indicated?

Answer 42

Strain/Substrain - Gene^Allele / +

Question 42

Describe genes carried in repulsion. How is this indicated?

Answer 42

Carried on different chromosome. Strain/Substrain - Gene1 +/+ Gene2^Allele

Question 43

Describe genes carried in coupling. How is this indicated?

Answer 43

Carried on same chromosome. Strain/Substrain - Gene1 Gene2^Allele /++

Question 44

How are recombinant congenic strains that have been backcrossed once or twice described differently than those that have been backcrossed more?

Answer 44

AcB vs AcB(N#)