NON-BLOOD SPECIMENS

Question 1

URINE
Type:
Collection?:
Preservation (tempt):
Container:
Processing: PCM

Answer 1

TYPE: Random, first morning, 8-hour
sample, timed sample, double voided,
24-hour sample
COLLECTION: Mid-stream, clean-catch,
catheter (culture) VOLUME: 30-60 mL
PRESERVATION: Refrigerator Temp (2-6
degrees Celsius)
CONTAINER: Screwcap Lid
PROCESSING: Within 2 hours
-Physical Examination (Color and
Clarity)
-Chemical Examination (SG, pH,
Glucose and Protein)
-Microscopic Examination (WBC,
RBC, Bacteria, Epithelial Cells)

Question 2

SEMEN
COLLECTION:
CONTAINER:
TEMPERATURE:
pROCESSING: VSMSW

Answer 2

COLLECTION: collected on-site
CONTAINER: Sterile, Wide-mouth container
TEMPERATURE: Must not be refrigerated
but kept as close to body temperature
PROCESSING: Within 2 hours
-Volume
-Sperm count, movement and shape, WHITE BLOOD CELL

Question 3

SPUTUM
COLLECTION:
VOLUME:
PROCESSING: GSA

Answer 3

COLLECTION: Deep respiratory secretions,
not saliva
VOLUME: about 1 teaspoon (5 milliliters)
PROCESSING: Immediately
-Gram Stain
-Sputum Culture
-AFB

Question 4

STOOL
COLLECTION:
VOLUME:
PROCESSING: PM

Answer 4

COLLECTION: Freshly Collected
VOLUME: 0.5 to 1.0 gram or pea-sized
PROCESSING: Within 2 hours
-Physical Examination (Color and
Consistency)
-Microscopic Examination (WBCs,
RBCs, Parasite, Yeast)

Question 5

BODY FLUID: CSF
SOURCE:
COLLECTION:
VOLUME

Answer 5

SOURCE: SPINAL SPACE
COLLECTION: LUMBAR PUNCTURE
VOLUME: > 0.5 mL(CLEAR)

Question 6

PLEURAL FLUID
SOURCE:
COLLECTION:
VOLUME

Answer 6

SOURCE: LUNGS
COLLECTION: THORACENTESIS
VOLUME: MINIMUM OF 50 ml

Question 7

PERITONEAL FLUID
SOURCE:
COLLECTION:
VOLUME

Answer 7

SOURCE: ABDOMINAL AREA
COLLECTION: PARACENTESIS
VOLUME: MINIMUM OF 50 ml

Question 8

PERICARDIAL
SOURCE:
COLLECTION:
VOLUME

Answer 8

SOURCE: SURROUNDING THE HEART
COLLECTION: PERICARDIOCENTESIS
VOLUME: MINIMUM OF 10 mL

Question 9

CSF, PLEURAL, PERITONEAL, PERICARDIAL
CONTAINER:
CENTRIFUGATION:
STORAGE:
ROUTINE TEST:

Answer 9

CONTAINER: glass and sterile
CENTRIFUGATION: 15 mins @ 2200-2500 RPM
STORAGE: Room tempt (7 days); Ref Tempt (7 days); Frozen Tempt (28 days)
ROUTINE TEST: GLUCOSE, TOTAL PROTEIN, WBC AND RBC COUNT, DIFFERENTIAL COUNT

Question 10

NASOPHARYNGEAL
WASH/ASPIRATE
OPTIMAL TIMING:
SPECIMEN COLLECTION:
SPECIMEN HANDLING:

Answer 10

OPTIMAL TIMING:
Within 3 days and not later than 7
days
SPECIMEN COLLECTION:
Insert tubing into the nostril parallel
to the palate.
SPECIMEN HANDLING:
Sterile vials @ 4 degrees Celsius