NonFermenting and Misellaneous Gram-Neg Bacilli Flashcards
(83 cards)
Most of the enteronacteriacae in this chapter are oxidase ___,
grow on ___ agar, and oxidize ___
oxidase positive,
grow on MacConkey agar, and oxidize glucose;
NONFERMENTING AND MISELLANEOUS GRAM-
NEGATIVE BACILLI
Withstand the treatments with
Chlorhexidine and Quartenary Ammonium Compounds
Agar used in biochemical test to determine fermenters and non fermenters
TSIA or Kligler iron agar (KIA)
A fermenter typically produces an ___ on TSIA or KIA within 18 hours of incubation
acid (yellow) butt with an acid or alkaline (red) slant
A nonfermenter produces __ in the butt and slant or may produce an _ slant.
no change in butt, alkaline (red) slant
Pseudomonas morphology
‘Gram-negative bacillus or coccopacilus
Pseudomonas motility
Motile, usually with polar flagellum or polar tuft of flagella
• blue phenazine pigment that contributes to the characteristic green color of P. aeruginosa, damages cells by producing reactive oxygen species.
Pyocyanin
pseudomonas are oxidase and catalase _
Positive
Pseudomonas fluorescent group, which includes P. aeruginosa, P. fluorescens, P. putida, P. veronii, P. mosselii, and P. monteilii produce
Pyoverdin
is water soluble and fluoresces under short-wavelength ultraviolet ligh
Pyoverdin
Most strains of P. aeruginosa will also produce the blue, water-soluble pigment called
Pyocyanin
other water soluble pigments occasionally produced I by strains of P. aeruginosa.
pyorubin (red) and pyomelanin (brown or black).
Other key characteristics of P. aeruginosa include :
- denitrification of _
-positive for _
- growth at _
- _ positivity
- _ utilization
- denitrification of nitrates to nitrite
-positive for r arginine dihydrolase (ADH) - growth at 42°C,
- citrate positivity
- acetamide utilization
is a selective and differential medium for the identification of P. aeruginosa.
Cetrimide agar
acts as a detergent and inhibits most bacteria; the medium also enhances the production of the two pigments produced by P. aeruginosa.
Cetrimide
No other nonfermentative, gram-negative
bacillus produces __, so its presence can be used to specifically identify P. aeruginosa
Pyocyanin
Pseudomonas has a strictly - metabolism
Aerobic
Acinetobacter spp. Appear as ___ or __ on gram stain
gram-negative coccobacilli or gram-negative cocci
Acinetobacter organisms can resist decolonization and retain the ___ leading to misidentification.
crystal violet stain
Acinetobacter spp. are plump coc-cobacilli that tend to resist alcohol decolorization, thus
sometimes appearing
Gram positive
Colonies of acinetobacter appear as Smooth, opaque, raised, creamy, and smaller than Enterobacterales;
some genospecies are beta-hemolytic on what agar
Trypticase soy agar with 5% sheep blood
The genus acinetobacter is divided into two groups
saccharolytic, asaccharolytic
Glucose oxidizing
saccharolytic
