Orefi

Question 1

What is the bevel angle in fine trimming?

Answer 1

27 - 32 degrees Celsius

Question 2

Why is cold wax better to cut for thinner sections?

Answer 2

Due to better support of harder elements

Question 3

What instrument is used to collect in fine trimming to complete ribbon sections

Answer 3

Camel hair or forceps

Question 4

Compare biconcave knives to wedge-shaped knives

Answer 4

Biconcave knives need smaller clearance angles compared to wedge-shaped knives

Question 5

What is the clearance angle in fine trimming ?

Answer 5

10 - 15 degrees Celsius

Question 6

What depends on the cutting rate in fine trimming?

Answer 6

Tissue type, block size and microtome model

Question 7

(3) reasons why Mayer’s egg albumin the most common choice in Tissue Adhesive Preparation?

Answer 7

Easy and convenient to prepare.

Inexpensive

A drop of the adhesive is applied on a clean glass slide before tissues are oriented

Question 8

This technique is used in Tissue adhesive preparation?

Answer 8

Pull Apart Technique

Question 9

What is the specimen used in tissue adhesive preparation?

Answer 9

Egg white

Question 10

What is the purpose of tissue adhesive preparation?

Answer 10

Promotes proper adhesion of tissue sections during processing.

Question 11

Why is accurate mounting and labelling important?

Answer 11

To preserve the specimen integrity

Question 12

List all the purpose of mounting

Answer 12

To preserve stained tissue sections permanently.

To protect the specimen from damage.

To enhance the optical quality under the microscope.

To allow for proper long-term storage and retrieval.

Question 13

What should be the result in fite faraco staining?

Answer 13

Blue-stained background with red/ magenta red-stained mycobacterium leprae bacilli

Question 14

What is the principle in fite faraco stain?

Answer 14

Targets the mycolic acid-rich cell walls of mycobacteria

Question 15

This system is used in reporting Pap smear

Answer 15

Bethesda system

Question 16

Why is thymol used in tissue adhesive preparation?

Answer 16

To prevent mold growth

Question 17

(4) Types of Hematoxylin

Answer 17

Alum Hematoxylin
Iron Hematoxylin
Copper Hematoxylin
Tungsten Hematoxylin

Question 18

Alum Hematoxylin Stain: Identify its ripening agent

1. Harris
2. Ehrlich’s
3. Gill’s
4. Delafield’s
5. Mayer’s
6. Cole’s

Answer 18

1. Mercuric oxide
2. Natural/Sodium iodate
3. Sodium iodate
4. Natural
5. Sodium iodate
6. Alcoholic iodine

Question 19

Enumerate the steps in H&E Staining

Answer 19

Deparaffinization/Dewaxing
Hydration
Primary stain + Mordant
Differentiation
Blueing
Counterstaining
Dehydration
Clearing
Mounting
Labelling