OSPE rabies

Question 1

case

Answer 1

agitation, photophobia, increased salivation and hydrophobia. The attendants gave the history of dog bite a month ago. She died within 2 weeks. The image provided is from her postmortem brain biopsy.

Question 2

Identify the findings in the stained smear

Answer 2

Post-mortem - Negri body, are eosinophilic , sharply outlined, pathognomonic intracytoplasmic inclusion bodies found in certain nerve cells esp at the hippocampus.

Question 3

Site of formation

Answer 3

In Hippocampus

Question 4

State the specimens that can help in antemortem diagnosis

Answer 4

-Serum:detection of antibodies
* Skin biopsy from hairline- Immunofluorescent assay can be performed for detection
of the viral antigens
* Corneal impression smear- Stained smears or Immunofluorescent assay

Question 5

Light and electron microscopic features of rabies in the
human brain.

Answer 5

A, Negri body in the cytoplasm of a Purkinje cell in the cerebellar cortex. (Hematoxylin & eosin.)

B, Rabies virions in the cytoplasm of a neuron are bullet-shaped(Electron microscopy.)