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Histopathology > OTHER COMMON STAINS > Flashcards

OTHER COMMON STAINS Flashcards

1
Q

– is a mixture of picric acid and fuchsin for the demonstration of connective tissues.

A

Acid Fuchsin-Picric Acid (Van Gieson’s Stain)

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2
Q

– is a basic acridine fluorochrome which permits discrimination between dead and living cells

A

Acridine Orange

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3
Q

– giving fluorescence for DNA and a red fluorescence for RNA.

A

Acridine Orange

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4
Q

– Is used to demonstrate deposits of calcium salts and possible sites of phosphatase activity.

A

Acridine Red 3B

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5
Q

– Is a complex, water-soluble phthalocyanin dye, similar to chlorophyll, which stains acid
mucopolysaccharides by forming salts linkages with them.

A

Alcian Blue

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6
Q

Alcian Blue is a complex, water-soluble phthalocyanin dye, similar to chlorophyll, which stains ________

A

acid mucopolysaccharides by forming salts linkages with them.

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7
Q

– Is a cytoplasmic stain used for counterstaining of epithelial sections.

A

Aniline Blue

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8
Q

– Is a plasma stain utilized also for deep staining of acid-fast organisms, for mitochondria, for
differentiation of smooth muscles with the used of picric acid

A

Basic Fuchsin

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9
Q

– It is main constituents of Feulgen’s (DNA) and Schiff’s reagent for the detection of aldehydes, Van Gieson’s solution for connective tissues, mucin, and for elastic tissue staining.

A

Basic Fuchsin

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10
Q

– Is used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for
staining diphtheria

A

Bismarck Brown

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11
Q

– Is usually combined with aluminum chloride to stain glycogen (Best Carmine Solution)

A

Carmine

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12
Q

– Mayer’s Carmalum Solution

A

Carmine

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13
Q

– Is a mordanted dye acting as a basic dye and staining acidic substances.

A

Carmine

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14
Q

– resistant to strong acid dyes

A

Celestine Blue

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15
Q

– recommended for routine staining of fixed sections, giving a good nuclear definition when used in conjunction with alum hematoxylin.

A

Celestine blue

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16
Q

– Is best known as an indicator, but may be utilized as a stain for axis cylinders embryos.

A

Congo Red

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17
Q

– it is used as a 4% aqueaous solution in Kajian’s method of staining elastic tissues, amyloid and
myelin

A

Congo red

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18
Q

– Is a nuclear or chromatin stain used for staining amyloid in frozen sections and platelets in blood .

A

Crystal Violet

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19
Q

– Crystal Violet + Methyl Violet + Dexterin = Gentian Violet

A

Crystal violet

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20
Q

– is used for staining blood to differentiates leukocytes

A

Giemsa Stain

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21
Q

– Gold sublimate

A

Giemsa Stain

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22
Q

– Is the stained used for metallic impregnation, made up of gold chloride and mercuric chloride

A

Giemsa stain

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23
Q

– It is used for microscopic study of starch granules

A

Giemsa stain

24
Q

– It is used for removal of mercuric fixative artifact pigments

A

Giemsa stain

25
Q

– Reagents to alter crystal and methyl violet so that they may retained by certain bacteria and fungi.

A

Giemsa stain

26
Q

– Is used for demonstrating mitochondria during intravital staining.

A

Janus Green B

27
Q

– It is used as a contrast stain for staining ascaris eggs and erythrocytes, and as a bacterial spore stain.

A

Malachite Green

28
Q

– It is also used both as a decolorizer and as a counter stain

A

Malachite green

29
Q

– Stains chromatin green in the presence of an acid.

A

Methyl Green

30
Q

– It gives false positive reaction with certain secretions as mucin

A

Methyl green

31
Q

– is a metachromatic dye formed whenever methylene blue is heated in fixed alkali carbonate

A

Methylene Violet

32
Q

coloring nuclei of leukocytes reddish-purple in the presence of methylene blue.

A

Methylene violet

33
Q

– Is a basic dye recommended for observing cell granules and vacuoles of phagocytic cell.

A

Neutral Red

34
Q

– Used as a substitute for carbol fuchsin in acid-fast staining

A

Night Blue

35
Q

– Is an excellent stain for elastic fibers (Tanzer Unna Orcein Method)

A

Orcein

36
Q

– especially recommended in dermatological studies

A

Orcein

37
Q

– a fixative and it is also used to stain fats

A

Osmium Tetroxide

38
Q

Fats reduces Osmium tetroxide into_______

A

Osmium dioxide (Black)

39
Q

– Contrast stain to acid fuchsin

A

Picric Acid

40
Q 
– Demonstration of connective tissue 
– Cytoplasmic stain 
– Counterstain to violet
– Fixative 
– Decalcifying agent
A

Picric acid

41
Q
  • Utilized to manufacture paints
A

Prussian Blue

42
Q
  • Microanatomical color contrast
A

prussian blue

43
Q
  • Demonstration of circulatory system by injection
A

Prussian blue

44
Q

– Used with osmic acid to fix and stain blood and glandular

– Silver Nitrate

A

Rhodamine B

45
Q

– Is used in 10% aqueous solution to prepare various dilutions to be used in identification of spirochetes, reticulum and other fiber stains

A

Rhodamine B

46
Q

– Is a nuclear stain for fixed tissues, used as a substitute for thionine in fresh frozen tissue sections

A

Toluidine Blue

47
Q

– It is recommended for staining of Nissl granules or chromophile bodies

A

Toluidine Blue

48
Q

– Is used for demonstration of neuroglia in frozen sections.

A

Victoria Blue

49
Q

OIL SOLUBLE DYES

A

LYSOCHROMES

50
Q

LYSOCHROMES

A
  • Sudan Black
  • Sudan Black B
  • Sudan IV
  • Sudan III
51
Q
  • most sensitive of the oil soluble dyes
A

Sudan Black

52
Q
  • greater affinity to phospholipids
A

Sudan Black

53
Q
  • stains Phospholipids and neutral fats
A

Sudan Black B

54
Q
  • do not stain crystalline cholesterol and free fatty acids
A

Sudan Black B

55
Q
  • does not color phospholipids, lipid droplets
A

Sudan IV

56
Q
  • recommended for triglycerides
A

Sudan IV

57
Q

good as a fat stain for CNS tissues

A

Sudan III

Histopathology (14 decks)

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