Oxidase Test Pg 71-72 Flashcards
The oxidase test identifies organisms that produce:
The enzyme cytochrome oxidase.
How does cytochrome oxidase participate in the electron transport chain?
By transferring electrons from a donor molecule to oxygen
The oxidase reagent contains:
A chromogenic reducing agent, which is a compound that changes color when it becomes oxidized.
If the test organism produces cytochrome oxidase, the oxidase reagent will turn ____ or ____ within 15 seconds.
Blue or purple
The organisms that contain cytochrome oxidase are _______. Do all organisms contain this enzyme?
Aerobic. No.
If it is _______________, it is an obligate aerobe and therefore does not ferment, which is an anaerobic process.
Oxidase +
Procedure: why can’t the oxidase disc be exposed to air until you are ready to start your experiment?
Exposure to air causes the oxidase reagent to react
What color indicates a positive oxidase result, in less than 30 seconds? … any longer = positive reaction is invalid as it reacts with air and water.
Purple color change
A negative result is what color change in under 30 sec?
No color change
