P1 34 Organisation Flashcards
(47 cards)
describe the chain of organisational levels
cell -> tissue -> organ -> organ system -> organism
function of the pancreas
- releases hormones (insulin+ glucagon) to help control blood sugar levels
- releases digestive enzymes (carbohydrase/ protease/ lipase) to aid food digestion
function of the gall bladder
stores bile
(helps fat digestion, neutralises stomach acid entering small intestine)
function of the kidneys
- filter waste from the blood
- gets rid of excess water+ salts from the body in the form of urine
function of the small intestine
- digestion- food is broken down (by digestive enzymes) and absorbed into blood
- releases the digestive enzymes (carbohydrase/ protease/ lipase)
adaptation of small intestine
walls lined with villi- aid absorption of nutrients by increasing small intestine’s surface area
adaptations of villi (3)
- microvilli - increase surface area
- walls 1 cell thick - short diffusion distance
- capillaries - good blood supply (for quick nutrient transport in+out) - maintains concentration gradient
function of the large intestine
- where indigestible food goes
- any water is absorbed+ the remaining food becomes faeces
4 functions of the liver
- removes toxins from the blood (to make bile)
- makes+releases bile - used to break down lipids into smaller molecules for faster digestion / neutralise stomach acid in small intestine so enzymes can work
- generates body heat
- stores glucose as glycogen when blood sugar concentration is too high
function of the oesophagus
- moves food to stomach by peristalsis- muscle contractions by rings of cartillage
function of the stomach
- chemical digestion- hydrochloric acid breaks down food+ harmful microbes
- physical digestion- churns food up
- provides acidic pH for Pepsin to work (break down protein into amino acids)
2 functions of bile +how it works
(made/stored)
- alkaline so neutralises acidic solution coming from stomach into small intestine- provides optimum pH for enzymes to work
- emulsifies fats to break them down into smaller droplets- increases surface area so lipase can work more efficiently
- released into the small intestine via bile duct
- made in liver
- stored in gall bladder
describe carbohydrase enzyme (eg. Amylase)
(made/works in)
- digests carbohydrates into simple sugars/glucose
- works in the mouth+ small intestine
- made in salivary glands+ pancreas+ small intestine
- Amylase breaks down starch into maltose sugar
describe protease enzyme
(made/works in)
- digests protein into amino acids
- works in stomach+ small intestine
- made in stomach+ pancreas+ small intestine
describe lipase enzyme
(made/works in)
- digests lipids (fats) into fatty acids+ glycerol
- works in small intestine
- made in pancreas+ small intestine
describe the test for starch
positive result- sample containing iodine solution changes from orange-brown to blue-black
describe the test for sugars
- add Benedict’s solution to a sample then place test tube in hot water bath
- positive result- sample changes from blue to green-yellow (low sugar level) or orange-red (high level)
describe the test for protein
positive result- sample containing Biuret solution changes from blue to purple
describe the test for lipids
positive result- sample containing ethanol+ equal volume of distilled water changes from clear to milky-white
describe what enzymes are + the 3 steps of lock and key theory
- a protein/chain of amino acids
- biological catalysts- speeds up a reaction without being used up
- can only complete one specific function - complementary shape allows them to fit together
- they break down large insoluble molecules into smaller soluble molecules
- an enzyme attracts substrates to its active site-> catalyses the chemical reaction-> forms smaller molecules
describe the effect of temperature on enzymes
- increase in temp -> increases rate of reaction (up to a certain amount)
- after around 41 degrees (above body temp) enzymes become denatured- enzyme’s active site can no longer bind with the substrate
- decrease in temp -> reaction is slow as enzymes have little kinetic energy so less frequent collisions w substrates
- optimum temperature (max rate of reaction) around 37 degrees
describe the effect of pH on enzymes+ examples
- enzymes become less effective or denatured if outside their specific optimum pH
- eg. pepsin (protease produced in stomach)- adapted to work in acidic conditions so stomach produces hydrochloric acid
- eg. bile- neutralises acidic solution coming from stomach so enzymes in small intestine can work in their preferred alkaline environment
describe the effect of concentration on enzyme reaction
- increase in concentration of substrate -> increased rate of reaction (up to a certain amount) as more substrate molecules available for enzymes to react
- after enzymes’ maximum rate achieved -> any increase in concentration has no effect on rate
state steps for effect of pH on enzymes RP
- place drops of iodine solution in rows on spotting tile
- label a test tube with the pH to be tested
- place 2cm³ amylase in test tube using a syringe
- add 1cm³ pH buffer solution to test tube using a syringe
- add 2cm³ starch solution to another test tube then add to amylase/buffer solution, start the stopwatch whilst mixing for 10 secs using a glass rod
- use a pipette to place one drop of the mixture on the first drop of iodine (should turn blue-black)
- wait another 10 secs and place another drop of the mixture on the second drop of iodine
- repeat every 10 secs until iodine solution no longer changes colour (remains orange-brown)
- repeat experiment at different pH values
-> the less time the iodine solution takes to remain orange-brown, the quicker the reaction, therefore the better the enzyme works at that pH