Paper 2 To Learn Flashcards

(29 cards)

1
Q

Why would a drug for pain receptors be injected directly into the cerebrospinal fluid rather than as a pill

A

-rapidly reaches spinal chord
-pill to large to absorb
-broken down by stomach Hydrochloric acid

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2
Q

When recording intensity of pain why is it important to use a statistically valid scale

A

-Pain tolerance is subjective
-To ensure that differences (in pain detection) were (statistically) significant for a valid comparison

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3
Q

Why would soil be sterilised before growth?

A

To remove
-pathogen
-pests
-competition
-saprobiants (nitrogen-fixing bacteria)

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4
Q

During an experiment seeing how volume of water affects growth why should soil contain the recommended amount of fertiliser?

A

-so water is the only variable being measured
-so ph of soil is not affected
-provide usual soil/farming conditions

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5
Q

How does colour vision allow orange to be seen?

A

Colour vision involves cone cells located in fovea on retina at back of eye
Photoreceptors contain pigments that absorb different wavelengths of light
Greater absorption by red-sensitive photoreceptors
More impulses sent to brain along optic nerve

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6
Q

Different processes leading to evolution of two species of one plant

A

Allopatric speciation
- geographical isolation
- Allopatric spectated due to the isolation / separation
- different selection pressures
- alleles given survival advantage increase in frequency in generations overtime
Sympatric speciation
- both found in similar locations
- variation due to mutation
- mutation leads to reproductive isolation separating gene pools by preventing interbreeding within a population eg. Different courtship
Different selection pressures

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7
Q

features of cells in proximal convoluted tubule to allow rapid re absorption of glucose into the blood

A
  • many microvilli to increase surface area
  • many carrier proteins for co-transport
  • many mitochondria produce ATP
  • many ribosomes to produce Carrier/ channel proteins
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8
Q

Where does ADH bind to in a nephron

A

Receptors on collecting duct and distal convoluted tubule

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9
Q

Where are receptors found that detect a decrease in blood pressure?

A

Aorta
Carotid artery

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10
Q

How does the release of ADH affect blood pressure

A

ADH increases re absorption of water
Increases volume of blood
Increases pressure

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11
Q

Ecosystems support a certain size of a population of a species called the …

A

Carrying capacity

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12
Q

Populations of different species will form a…

A

Community

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13
Q

Reasons for low efficiency of energy transfer from secondary consumers to tertiary consumers in an ecosystem

A

-not all food digested
-not all parts of animal eaten
-excretion
-heat loss from respiration

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14
Q

If P < 0.05 what does this show

A
  • there is a significant difference
  • results not due to chance
  • reject null hypothesis
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15
Q

How could a sequence of DNA be removed from a DNA sample?

A
  • restriction endonuclease
  • cut dna at a specific base sequence
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16
Q

Why is is important to know base sequences in a PCR?

A

for primers, to produce complementary base sequences

17
Q

PCR amplification formula

18
Q

What features allow base sequences to be separated by gel electrophoresis ?

A

Number of nucleotides / bases
Negative charge

19
Q

Role of ATP in muscle contraction

A
  • binding of ATP to myosin head breaks actin-myosin cross bridge
  • causes myosin head to detach and this moves the head
  • this causes actin filaments to move inwards
  • ATP used for active transport of calcium ions back into sarcoplasmic reticulum
20
Q

In sex linked inheritance when an allele is carried on the X chromosome causes a disorder why are males more likely to have the disorder?

A

Males will only have one allele
Females need two recessive alleles / must be homozygous recessive
Females could be heterozygous and carriers

21
Q

Codominance

A

Codominance is a type of inheritance pattern where both alleles in a heterozygous individual are fully and equally expressed.

22
Q

In genetic crosses, the observed phenotypic ratios obtained in the offspring are often not the same as the expected ratios. Explain why

A
  • small sample size
  • random fusion of gametes
  • linked genes
  • Epistasis
23
Q

Explain why there is a wide variation in the patterns of repeated, non-coding sequences (VNTRs).

A

VNTRs are non-coding.

This means that a change in the number of repeats does not have any impact on an organism’s phenotype.

As a result, natural selection does not act on variation in the number of repeats in VNTRs.

Individuals with all patterns of repeats pass them on to their offspring and mutation leads to even more new variations.

24
Q

During electrophoresis why are bands transferred to membrane / paper?

A

Agarose gel is not very durable
Risk of bands becoming damaged
Transferal preserves them

25
How do you make the bands easier to see during gel electrophoresis?
Add RNA probes with stain - colour will show / will become fluorescent under UV light
26
Process of PCR
-chain reaction mixture of DNA sample, free nucleotides, primers and taq polymerase -heat to 95°c to allow the hydrogen bonds between complementary base pairs to break allowing strands to separate -cool to 55°c to allow primers to bind to the separated strands -heated to 72°c as this allows the dna polymerase to work effectively -dna polymerase (taq) catalyses the formation of complementary dna strands -> replicating the molecule
27
Why does the DNA need to be amplified by PCR before genetic fingerprinting?
Blood sample will only contain very small amounts of DNA Amplification needed to provide enough DNA for the bands produced by genetic fingerprinting to be visible
28
Why do DNA samples from different individuals produce different patterns of bands when genetic fingerprinting is performed?
Genome contains different areas of of VNTRs Different individuals have different numbers of repeats at different loci DNA fragments corresponding to different numbers of repeats are separated according to size Patterns of bands produced corresponds to the number of repeats an individual has at each locus
29
Explain how you would determine if the genotype of a grey fly is homozygous or heterozygous for body colour.
Cross it with a homozygous recessive black fly (gg) If there is black / black + grey offspring it will be heterozygous (Gg) No black offspring homozygous (GG)