Para And Ento Techniques Flashcards
1
Q
Radiolabelling
A
Tagging of Antigen with 3H 14C 125I
2
Q
Preparation of Specific Antibody
A
Antigen is injected into the animals body
Antibodies are recovered the serum
3
Q
Development of the Assay System
A
Bounded antibodies and antigen is placed on the microtitre wall surface
Decanting and washing will remove the unbound antigen
4
Q
Types of ELISA
A
Competitive binding assay
Non-competitive binding assay
5
Q
Continuation of the Assay Procedure
A
After decantation and all the unbound antigens are removed
Radioactivity remaining in the microtitre well is by the GM or Scintillation counter
Intensity of radiation is inversely correlated with antigen concentration
