Stains And Staining Method Flashcards
In the diagnosis of enteric parasites relies on
Microscopic examination of stained specimen
Stain Quality
Staining is important for two reasons
Accurate identification and diagnosis
Preservation
Which stain helps in differentiating blood cells types, also a mixture of methylene blue dye
Wright stain
Non specific stain used for parasitic cysts
Lugol’s iodine
Immunodiagnostic method
RIA
ELISA
Immunofluorescence assay
Flow cytometry
ELISA differentiate from RIA due to their
Detection Method
In ELISA detection is based on
Enzyme catalysed reaction
Fluorescent probe
Which immunodiagnostic method can be used for both HIV testing and DRUG monitoring
ELISA
Another name for non-competitive binding is
Sandwich method
Which stain is best suited for staining blood parasites like malaria and microfilaria
GIESMA stain
What is needed for preparation of Giemsa Stain (for 500mls)
Giemsa stain powder 3.8g
Methanol 250mls
Glycerol 250mls
Glass beads
Principle of GIESMA
The ‘neutral’ dyes combining the basic dye (methylene blue) and the acid dye (eosin), give a wide color range when staining
Principle of field staining
The field stain B (eosin) gives a wide color range while staining
The difference between giemsa and field stain is
Field stain is water- based and is also a mixture of Methylene blue (stain A) and eosin (stain B)
What is the process of preserving biological materials
Fixation
