PARASIT BASICS LAB

Question 1

Most common fixative for preservation in Parasit

Answer 1

10% formalin
polyvinyl alcohol

Question 2

All purpose fixative
has 1:3 stool to fixative ratio

Answer 2

formalin

Question 3

Formalin concentration for protozoan cyst

Answer 3

5%

Question 4

concentration of formalin for helminth eggs and larvae

Answer 4

10%

Question 5

Very useful for field survey fixative
disadvantage contains mercuric chloride

Answer 5

Merthiolate Iodine Formaldehyde

Question 6

Considered as the gold standard solution
permanent stain smear or execellent preservation of protozoan trophozoites and cysts

Answer 6

Schaudinn’s solution
Mercury based

Question 7

Eco friendly fixative

Answer 7

Modified polyvinyl alcohol
copper or zince

Question 8

Macroscopic examination includes

Answer 8

color
consistency
adult worms, proglottids
pus
mucus, blood

Question 9

Microscopic examination includes

Answer 9

Parasites and ova

Question 10

Preparation for microscopic

Answer 10

direct wet preparation
concentrated technique (fixed/fresh)
Permanently stained smear

Question 11

Ocular micrometer

Answer 11

disk inserted to the eyepiece
size is important
measured as microms

Question 12

best technique for the recovery of Schistosoma, opeculated eggs, trematode eggs, cestode eggs

Answer 12

Sedimentation technique
FEACT

Question 13

Dissolves neutral fats/lipids and CHO

Answer 13

ether/ethyl acetate

Question 14

best technique for the recovery of Protozoan cyst, nematode

Answer 14

Flotation
main reagent: 33% zinc sulfate
spx: stool

Question 15

specific gravity range in floatation in zinc sulfate solution

Answer 15

1.18-1.20

fresh stool: 1.18
fixed stool 1.20

Question 16

Best floatation technique for cryptosporodium, cyclospora

Answer 16

Sheathers sugar floatation
not the method of choice for helminths

Question 17

modified trichrome color change

Answer 17

spores are pink to red green background

Question 18

egg counting procedure that uses sieved and wire mesh

Answer 18

Kato katz

Question 19

recommended method of WHO useful in assessing intensity of infection of STH and unholy three

Answer 19

Cellophane covered smear

Question 20

thin film for malaria

Answer 20

confirmatory
malaria id

Question 21

thick film for malaria

Answer 21

screening
quantifying malarial parasite

Thick and thin smear is the gold standard

Question 22

parasites recovered in blood

Answer 22

Leishmania donovani
Trypanosoma spp
Plasmodium
Babesia
Microfilariae

1hr if malaria is suspected

Question 23

CSF PARASITE

Answer 23

Naegleria fowleri and Acanthamoeba
Trypanosoma spp - sleeping disease

Question 23

Rectal biopsy specimen parasite

Answer 23

Schistosoma spp

Question 24

Skin biopsy skin snips

Answer 24

Onchocerca volvulus Mansonella

Question 25

Sputum

Answer 25

ASH Ascaris Strongyloides Hookworm

Question 26

Eye specimens

Answer 26

Acanthamoeba keratitis T. gondii Loa loa

Question 27

Mouth scrapings

Answer 27

Entamoeba gingivalis Trichomonas tenax

Question 28

Nasal discharge

Answer 28

Naegleria fowleri

Question 29

Gold standard for parasitic examniation diseases

Answer 29

Microscopic examination

Question 30

when fixative is not an option, stool can be mixed in ________

Answer 30

Potassium dichromate 1:1 Absolute ethanol 1:1 stored at 4C frozen with dry ice