part2

Question 1

Import of proteins into which one of the following organelles is described as “gated” – a) lysosome, b) peroxisome, c) nucleus, d) mitochondrion?

Answer 1

Nucleus

Question 2

If a polypeptide possesses an internal sequence of PKKKRKV, which organelle is it most likely to be targeted to?

Answer 2

Nucleus

Question 3

Which one of the following proteins is not originally synthesised with a leader sequence – a) catalase, b) albumin, c) citrate synthase, d) a-galactosidase?

Answer 3

Catalase

Question 4

Which one of the following techniques can be used to study proteins –
a) western blotting, b) northern blotting, c) southern blotting, d) eastern blotting?

Answer 4

Western Blotting

Question 5

Which two of the following are the most commonly used reagents to visualize proteins on SDS-PAGE gels – a) Coomassie Blue, b) Texas Red, C) silver nitrate, d) gold chloride?

Answer 5

Coomassie blue

Question 6

Name a plasma membrane phospholipid that is usually entirely restricted to the inner leaflet in healthy cells.

Answer 6

Phosphatyidylserine

Question 7

To which class of lipids does sphingomyelin belong?

Answer 7

Sphingolipids

Question 8

Which one of the following proposed the signal hypothesis for protein trafficking – a) George Palade, b) James Rothman, c) Gunter Blobel, d) Christian De Duve?

Answer 8

Gunter Blobel

Question 9

At which intracellular site does the AP2 adaptor complex function – a) TGN,
b) plasma membrane, c) ER, d) lysosome?

Answer 9

Plasma Membrane

Question 10

Which one of the following processes do SNARES facilitate - a) membrane fusion, b) nuclear import, c) protein synthesis, d) post-translational modification?

Answer 10

Membrane Fusion

Question 11

Which one of the following organelles has the lowest pH – a) peroxisomes, b) mitochondria, c) lysosomes, d) chloroplasts?

Answer 11

Lysosomes

Question 12

The ced genes are important for controlling which two of following processes – a) apoptosis, b) differentiation, c) final cell numbers, d) necrosis?

Answer 12

apoptosis and final cell numbers

Question 13

Which two of the following are properties of stem cells - a) self renewal, b) can give rise to progenitor cells, c) postmitotic, d) only found in bone marrow?

Answer 13

Self Renewal, Can give rise to progenitor cells

Question 14

Give the name of two members of the Cys-loop receptor family.

Answer 14

GABA and glycine

Question 15

Starting with G1 place the four phases of the cell-cycle in the correct order.

Answer 15

G1, S, G2, M

Question 16

Name two ubiquitin ligases important in regulating the cell-cycle.

Answer 16

APC/C, SCF

Question 17

Name the two CDKs that the INK4 family inhibit?

Answer 17

Cdc4 and Cdc6

Question 18

Name the two main groups that bacteria can be divided into.

Answer 18

Archabacteria and eubacteria

Question 19

What is the structure called that bacteria use to swim through liquids?

Answer 19

Flagella

Question 20

What is the name of the rigid polymer that surrounds bacterial cells?

Answer 20

Lipopolysaccharide

Question 21

Name two intracellular structures that require dyneins.

Answer 21

Cilia, Golgi Body

Question 22

Name two agents that disrupt F-actin.

Answer 22

Phallodin, Jasplakinolide

Question 23

Name two components of the extracellular matrix.

Answer 23

Collagen, Proteoglycan

Question 24

What is the name given to the evolutionary process by which eukaryotic cells are thought to have acquired mitochondria?

Answer 24

Symbiogenesis

Question 25

What is another word for programmed cell death?

Answer 25

Apoptosis

Question 26

Isopycnic or equilibrium density gradient centrifugation is used to separate intracellular organelles on the basis of which one of the following properties – a) size, b) shape, c) density, d) pH?

Answer 26

Density

Question 27

The IP3 receptor, the cAMP gated channel, and the cGMP gated channel can all be gated by which one of the following - a) extracellular solute, b) intracellular solute, c) membrane voltage, d) membrane lipid composition?

Answer 27

Extracellular solute

Question 28

What is the name given to the process that involves multiple rounds of DNA replication without cell division?

Answer 28

Endoreduplication

Question 29

What is the name given to the microtubule organizing centre in a eukaryotic cell?

Answer 29

centrosome

Question 30

Which one of the following is the main cytoskeletal component of the mitotic spindle – a) actin filaments, b) intermediate filaments, c) microtubules, d) microfilaments?

Answer 30

Microtubules