PBS AND COUNTING

Question 1

• provide valuable information regarding px health

Answer 1

pbs and counting

Question 2

preferred edta tube

Answer 2

liquid tripotassium edta

Question 2

evacuated tubes used in collection

Answer 2

anticoagulant-free blood
lavender topped tubes (containing dipotassium or tripotassium edta)

Question 2

capstone of a panel of test is called

Answer 2

cbc or histogram

Question 2

t/f: after collection, it must be made within 24 hours of drawing the specimen

Answer 2

f - dapat 4 hrs lang

Question 3

• in vitro phenomenon
• platelets adhere to neutrophils
• causes pseudothrombocytopenia in automated methods

Answer 3

platelet satellitosis

Question 4

result of platelet satellitosis in PLT and WBC count

Answer 4

low platelet count
false increase wbc count

Question 5

what tube is used to correct plt satellitosis

Answer 5

sodium citrate tube

Question 6

ratio of sodium citrate tube

Answer 6

9:1

Question 6

made immediately at the px’s side
disadvantages: plt clumping, few films can be made directly

Answer 6

finger and heel punctures

Question 7

easiest to master
most convenient and commonly method used

Answer 7

manual wedge method

Question 7

size of glass slide, drop of blood and angle

Answer 7

glass slide: 3x1 inch
blood: 2-3mm in dm
angle: 30-45 degrees

Question 8

makes the cell more visible and to allow their morphology to be evaluated

Answer 8

staining

Question 9

stains pbs and bone marrow smears

Answer 9

pure wright stain/ giemsa-wright stain (romanowsky stain)

Question 10

dye used in polychrome (wright and leishman) stain

Answer 10

eosin & methylene blue

Question 10

dye used in giemsa stain

Answer 10

eosin, azure b, methylene blue

Question 10

fast and easy
the whole process takes about 1 minute

Answer 10

quick stain

Question 11

used as buffer in quick stain

Answer 11

aged distilled water

Question 12

• performed over a sink or pan with a staining rack
• strains: filtered before use or poured from the bottle

Answer 12

manual technique

Question 13

in manual technique, stains must be left on the slide for how many minutes

Answer 13

1-3 minutes

Question 14

this is an indicator that the mixing is correct

Answer 14

metallic sheen or green scum

Question 15

t/f: best results are obtained on fresh slides

Answer 15

t

Question 15

rbc appearance after staining

Answer 15

appears orange to salmon pink

Question 16

neutrophil appearance after staining

Answer 16

cytoplasm should be pink to tan with violet or lilac granules

Question 16

wbc appearance after staining

Answer 16

nuclei turned purple to blue

Question 16

eosinophil appearance after staining

Answer 16

bright orange refractile granules

Question 17

ideal area of viewing in pbs

Answer 17

between the feathered edge and thick region

Question 17

performed in a systematic manner

Answer 17

differential counting

Question 18

this pattern is preferred because it minimizes distribution errors and ensures that each cell is counted once

Answer 18

back-and-forth serpentine or battlement track pattern

Question 19

push-down counters or newer computer-interfaced key pads

Answer 19

counters

Question 20

t/f: results are reported as tenths

Answer 20

f -percentage