PCR design Flashcards
(9 cards)
1
Q
What does reverse transcriptase do?
A
Converts RNA to cDNA
2
Q
Why include start and stop codons in PCR?
A
For full protein expression
3
Q
Why is H2O added last?
A
To make up the volume to desired amount
4
Q
Primer direction?
A
Forward from start codon
Reverse from stop codon
5
Q
Why do PCR setup on ice?
A
Prevent premature enzyme activity
6
Q
Why vortex buffer in PCR?
A
Evenly mixes Mg2+ ions throughout
7
Q
Where would you find the gene sequence?
A
NCBI using accession number
8
Q
Why avoid bubble in a PCR?
A
They obstruct heat transfer
9
Q
Why use a mastermix?
A
Reduces pipetting errors.
