Pecori 2022 Apobec Functions Flashcards
(22 cards)
A3a functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- Rna and dna in viruses and hosts
- N and C
- innate immune cells - macros monos microglia and dendritic cells
- 5-TC-3
- protection form viruses
A3g functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host rna and viral rna&dna
- cytoplasm
- all over - breasts, reproductive organs, IFNa activates cells, bone marrow
- 5-CC-3
- protection from viruses and retro elements
A1 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host rna&dna and viral rna
- N and C
- GI tract in human and mouse and immune cells in mice only
- 5’AC’3
- cholesterol metabolism, monocyte transcriptome (ms only), antiviral
Aid functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ssDNA of hosts
- N and C
- germinal center B cells, lymphoid tissue
- 5-wrc-3
- secondary antibody diversification
A2 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- host dna ss/Da binding
- N and C
- muscle and some B/T cells
- ND
- ND
A3b functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- dna of viruses and hosts
- nucleus
- ifna activated liver cells, bone marrow, gi tract, kidney and bladder
- 5-TC-3
- protection from viruses
A3c functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Nucleus and Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and lots more
- 5-TC- 3
- protection from viruses
A3d functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and more
- 5-TC- 3
- protection from viruses
A3f functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm
- peripheral blood cells, bone marrow and lymphoid tissue and more like ifna cells
- 5-TC- 3
- protection from viruses
A3h functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?
- ss virus dna
- Cytoplasm and nucleus
- peripheral blood cells, bone marrow and lymphoid tissue
- 5-TC- 3
- protection from viruses
what are the generalist apobecs?
a3a a3g and a1
what part of generalists determines substrate differentiation?
loops 1 3 5 7 determine binding and single AAs determine RNA and or DNA binding ability
what structure do generalists all have in common?
they all have a ‘u’ shaped groove where substarte binding occurs
which apobecs have nuclear localization signal domains?
aid a1 and a3b
which apobecs have nuclear export signal domains?
aid and a1
where is the zn binding domain located?
under loop 1 and connected between loop 3 and loop 5
a2 prefers to bind to ssDNA where? what function does this serve?
in GC rich motifs (typically seen in promoter regions)
regulation of gene transcription (usually through repression from promoter binding)
single domain A3’s and double domain A3’s
single - a3a, a3c, a3h
double - a3b, a3d, a3f, a3g
Double domain A3s have difficulties with what?
entering the nucleus
what apobec can passively enter the nucleus but does not contain a NLS?
A3h
what apobec remains in the cytoplasm and why?
A3G
It contains a novel CRS (cytoplasmic retention signal)
A secondary Zn2+ was founf in which apobecs
A3A and A3G
