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APOBEC > Pecori 2022 Apobec Functions > Flashcards

Pecori 2022 Apobec Functions Flashcards

1
Q

A3a functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • Rna and dna in viruses and hosts
  • N and C
  • innate immune cells - macros monos microglia and dendritic cells
  • 5-TC-3
  • protection form viruses
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2
Q

A3g functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • host rna and viral rna&dna
  • cytoplasm
  • all over - breasts, reproductive organs, IFNa activates cells, bone marrow
  • 5-CC-3
  • protection from viruses and retro elements
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3
Q

A1 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • host rna&dna and viral rna
  • N and C
  • GI tract in human and mouse and immune cells in mice only
  • 5’AC’3
  • cholesterol metabolism, monocyte transcriptome (ms only), antiviral
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4
Q

Aid functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • ssDNA of hosts
  • N and C
  • germinal center B cells, lymphoid tissue
  • 5-wrc-3
  • secondary antibody diversification
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5
Q

A2 functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • host dna ss/Da binding
  • N and C
  • muscle and some B/T cells
  • ND
  • ND
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6
Q

A3b functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • dna of viruses and hosts
  • nucleus
  • ifna activated liver cells, bone marrow, gi tract, kidney and bladder
  • 5-TC-3
  • protection from viruses
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7
Q

A3c functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • ss virus dna
  • Nucleus and Cytoplasm
  • peripheral blood cells, bone marrow and lymphoid tissue and lots more
  • 5-TC- 3
  • protection from viruses
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8
Q

A3d functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • ss virus dna
  • Cytoplasm
  • peripheral blood cells, bone marrow and lymphoid tissue and more
  • 5-TC- 3
  • protection from viruses
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9
Q

A3f functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • ss virus dna
  • Cytoplasm
  • peripheral blood cells, bone marrow and lymphoid tissue and more like ifna cells
  • 5-TC- 3
  • protection from viruses
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10
Q

A3h functions
- rna/dna in virus or host?
- localized to?
- tissue expression examples?
- target seq.?
- known biological functions?

A
  • ss virus dna
  • Cytoplasm and nucleus
  • peripheral blood cells, bone marrow and lymphoid tissue
  • 5-TC- 3
  • protection from viruses
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11
Q

what are the generalist apobecs?

A

a3a a3g and a1

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12
Q

what part of generalists determines substrate differentiation?

A

loops 1 3 5 7 determine binding and single AAs determine RNA and or DNA binding ability

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13
Q

what structure do generalists all have in common?

A

they all have a ‘u’ shaped groove where substarte binding occurs

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14
Q

which apobecs have nuclear localization signal domains?

A

aid a1 and a3b

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15
Q

which apobecs have nuclear export signal domains?

A

aid and a1

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16
Q

where is the zn binding domain located?

A

under loop 1 and connected between loop 3 and loop 5

17
Q

a2 prefers to bind to ssDNA where? what function does this serve?

A

in GC rich motifs (typically seen in promoter regions)

regulation of gene transcription (usually through repression from promoter binding)

18
Q

single domain A3’s and double domain A3’s

A

single - a3a, a3c, a3h
double - a3b, a3d, a3f, a3g

19
Q

Double domain A3s have difficulties with what?

A

entering the nucleus

20
Q

what apobec can passively enter the nucleus but does not contain a NLS?

A

A3h

21
Q

what apobec remains in the cytoplasm and why?

A

A3G
It contains a novel CRS (cytoplasmic retention signal)

22
Q

A secondary Zn2+ was founf in which apobecs

A

A3A and A3G

APOBEC (3 decks)

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