PHLEB: Color Coding for Tube Tops

Question 1

RED TOP TUBE

Answer 1

No Additive (glass tube)

Clot Activator (plastic tube)

Question 2

Inversions for Red Top Tube

Answer 2

0 inversions (glass tube)

5 inversions (plastic tube)

Question 3

Sample for Red Top Tube

Answer 3

Serum

Question 4

Blood Clotting time for Red Top Tube

Answer 4

60 minutes

After collecting the serum, let the sample stand by at room temperature for 30-60 minutes before centrifuge

Question 5

Lab uses for Red Top Tubes

Answer 5

Serum determinations in chemistry

Blood donor screening (crossmatching)

Diagnostic testing for infectious diseases

Question 6

Lab sections that use Red Top Tubes

Answer 6

Clinical Chemistry

Blood Bank

Immunoserology

Question 7

GOLD OR MOTTLED-RED-GRAY TOP TUBES

Answer 7

clot activator

thixotropic gel separator (SST)

Question 8

Sample for Mottled-Red-Grey Top Tube

Answer 8

Serum

Question 9

Blood clotting time for Mottled-Red-Grey Top Tube

Answer 9

30 minutes

Question 10

Lab uses for Mottled-Red-Grey Top Tube

Answer 10

Serum determination in chemistry

Blood donor screening (crossmatching)

Serum testing for infectious diseases

Question 11

Inversions for Mottled-Red-Grey Top Tube

Answer 11

5 inversions

Question 12

Tubes that sample serum

Answer 12

Red

Yellow

God or Mottled-Red-Gray

Question 13

LIGHT BLUE TOP TUBE

Answer 13

Anticoagulant: 3.2 % sodium citrate

Question 14

Sample for Light Blue Top Tube

Answer 14

Plasma

Question 15

Inversions for Light Blue Top Tube

Answer 15

3-4 gentle inversions

Question 16

Lab uses for Light Blue Top Tube

Answer 16

Clot-based/coagulation studies (PT and APTT)

mixing studies

ethanol gelation test

Question 17

Lab sections that use Light Blue Top Tube

Answer 17

Hematology

Question 18

GREEN TOP TUBE

Answer 18

Anticoagulant: heparin

Lithium heparin
Ammonium heparin
Sodium heparin

Question 19

Sample for Green Top Tube

Answer 19

Arterial Blood

Question 20

Inversions for Green Top Tube

Answer 20

8 full inversions

Must be full and transported on ice

Question 21

Lab Uses for Green Top Tube

Answer 21

Plasma Determinations in Chemistry

Question 22

PURPLE TOP TUBE

Answer 22

Anticoagulant: EDTA

Spray-coated K3EDTA (glass)
Liquid K2EDTA (plastic)

Question 23

Sample for Purple Top Tube

Answer 23

plasma (blood bank or immunohematology)
whole blood (hematology)

Question 24

Inversions for Purple Top Tube

Answer 24

8-10 full inversions

Question 25

Lab use for Purple Top Tube

Answer 25

Hematology determinations (CBC) Routine immunohematology testing Blood donor screening (crossmatching)

Question 26

Only test in Clinical Chemistry that uses Lavender Top

Answer 26

HBA1C

Question 27

GREY-TOP TUBE

Answer 27

Anticoagulant: potassium oxalate monohydrate Antiglycolytic : sodium fluoride

Question 28

Sample for Grey-Tope Tube

Answer 28

Plasma and Whole blood

Question 29

Inversions for Gray Top Tube

Answer 29

8-10 full inversions

Question 30

Lab uses for Gray Top Tube

Answer 30

glucose determinations lactic acid level determination

Question 31

YELLOW TOP TUBE

Answer 31

Acid Citrate Dextrose Sodium Polyanethol Sulfonate

Question 32

Lab uses for Yellow Top Tube

Answer 32

ACD: Blood bank studies HLA phenotyping Paternity testing DNA SPS : Used for special blood culture studies Inhibits certain antibiotics

Question 33

Inversions for Yellow Top Tube

Answer 33

8 full inversions

Question 34

Sample for Yellow Top Tube

Answer 34

Plasma and Whole Blood

Question 35

ORDER OF DRAW

Answer 35

Blood Culture or sterile tubes (yellow) Coagulation tube (light blue) Serum tube (red or gold) Heparin tube (green or light green) EDTA tube (lavender) Oxalate/fluoride tube (gray)

Question 36

Importance of Order of Draw

Answer 36

To prevent cross-contamination of samples To prevent transfer of tissue thromboplastin To prevent additive carry-over To prevent contamination with microorganisms

Question 37

Order of Draw memory jogger

Answer 37

Stop (sterile) Light (light blue) Red (red) Stay (SST) Put (PST) Green (Green) Light (Lavender) Go (Gray)