Phlebotomy: colors, additives, uses, operations Flashcards

1
Q

Explain the mnemonic:

Stop-Light-Red

Stay-Put

Green-Light-Go

A
  • Stop – Sterile (yellow)
  • Light – Light blue tube
  • Red – Red top tube
  • Green – GTT (Green top tube)
  • Light – LTT (Lavender top tube)
  • Go – (Gray-top tube)
  • Stay – SST/Red gray (Serum separated tube)
  • Put – PST/Lt green (Plasma separated tube)
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2
Q
  1. ) Three methods of drawing blood
  2. ) Capjet use method for infants
A

Three methods of blood draw

  • Vacutainer method/needle
  • Butterfly method/syringe-needle
  • Fingerstick/milking

Capjet method for infants

  • Heel stick, collect a drop, mix, repeat
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3
Q
  1. With an MI, collect a blood sample and _____.
  2. One should always draw above or below the IV line?
A
  1. Centrifuge, do not wait for it to clot
  2. Below
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4
Q

Additives in yellow-top tubes and their function

A

Sodium polyethanol sulfate (SPS)

Blood culture specimen collection in microbiology

Acid citrate dextrose (ACD) A or B

Blood bank studies

HLA phenotyping

DNA Paternity testing

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5
Q

Additive in blue-top tubes

A
  1. Buffered sodium citrate
  2. Citrate, theophylline, adenosine dipyridamole (CTAD)
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6
Q

Laboratory use of light blue tubes

A
  • Routine coagulation determination
  • CTAD for platelet function assays
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7
Q

What is the additive in red-top tubes?

What is the laboratory use of red-top tubes?

A

Clot activator (plastic siding)

Laboratory use (60 sec to clot):

  1. Chemistry serum determination
  2. Blood donor screening
  3. Infectious disease screening
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8
Q
  • Orange tube additive
  • Laboratory uses of orange-top tubes
A
  • Thrombin clot activator (5 minutes)
  • Chemistry stat serum determinations
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9
Q

Additives in gold or red/gray top tubes

Laboratory use of red/gray top tubes

A
  • Clot activator
  • Gel for serum separation
  • Chemistry serum determination
  • Routine blood donor screening
  • Diagnostic testing for infectious disease
  • Clotting time = 30 minutes
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10
Q

Light green/gray-tube

  • Additives
  • Laboratory Use
A

Additives

  • Lithium heparin
  • Gel for plasma separation

Laboratory use

  • Chemistry plasma determination
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11
Q

Green top tube additives

A

Sodium heparin

Lithium heparin

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12
Q

Green top tube laboratory use

A

Chemistry plasma determination

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13
Q

Lavender top tube additives (2)

A

Liquid K3 EDTA

K2 EDTA

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14
Q

Pink top tube additives

A

K2 EDTA

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15
Q

White top tube additives and laboratory use

A

K2 EDTA and gel for plasma separation

  • Molecular diagnostic test methods:
  • PCR and/or branched DNA (bDNA)
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16
Q

Lavender and pink top tube laboratory use

A

Whole blood hematology determination

K2 EDTA used for

  • Immunohematology testing
  • Blood donor screening
17
Q

Gray top tube additives

A
  • Sodium flouride + Potassium oxalate
  • Sodium flouride + Na2 EDTA
  • Sodium fluoride
18
Q

Gray top tube laboratory use

A

Glucose determination

  • Oxalate and EDTA will give plasma samples
  • Sodium flouride is the antiglycolytic agent
19
Q

Royal blue additives

A

Clot Activator

K2 EDTA

20
Q

Royal blue laboratory use

A
  1. Nutritional chemistry determinations
  2. Trace elements
  3. Toxicology
21
Q

Tan top tube additives

A

K2 EDTA

22
Q

Tan top tube laboratory use

A

Lead determination

Certified to contain less than .01 ug/mL (ppm) lead

23
Q

Clear red/gray top tube additives

Clear red/gray top tube laboratory use

A

None

Discard or secondary specimen

24
Q

Tubes of particular importance

A

Red (general, bloodbank)

Light Blue (coagulation)

Lavender (diff, cbc)

25
Q

What do you do if:

  1. Patient is not bleeding from needle removal
  2. Patient bleeds from needle removal
A

IMMEDIATELY cap/dispose of needle. Attend patient.

Attend patient. Cap needle. DISPOSE of needle.