Photosynthesis

Question 1

Paper Chromatography of Plant Pigments

Answer 1

Photosynthetic pigments can be separated by paper chrom

Pigments extracted from a leaf and a conc spot is created on chrom paper

Diff pigments identified by colour and positions

Rf value calculated

Question 2

Redox Indicators role of H acceptors in PS

Answer 2

During light dependant stage electrons energised to reduce NADP

Using DCPIP to accept e demonstrates activity of LD stage experimentally

DCPIP reduced turns from blue to colourless

Question 3

Demonstrate activity of LD stage PS

Answer 3

DCPIP added to a suspension of chloroplasts - time taken to decolourise measured

Loss of colour due to electrons produced by LD reactions in isolated chloroplasts

A control in which DCPIP is added to water would remain blue