Respiration

Question 1

Respiratory Quotient

Answer 1

RQ= vol CO2 produced
vol O2 consumed

Question 2

Respirometer for volume Oxygen

Answer 2

Volume oxygen consumed by living organisms determined

Potassium Hydroxide to absorb the CO2 produced

Question 3

Respirometer for volume Carbon Dioxide produced

Answer 3

Respirometer with water replacing the potassium hydroxide

Measures net diff between CO2 produced and O2 consumed

Using vol O2 consumed from prev u can calculate vol CO2 prod

Question 4

What do RQ values provide

Answer 4

Clues to the substrate being respired and the extent to which the tissue is respiring anaerobically

Question 5

Redox Indicators

Answer 5

Artificial Hydrogen acceptors which change colours when reduced

Added to suspension of living material and observations made of colour change
-how long it takes for colour to disappear
-colorimeter - % transmission increase as solution loses blueness

Question 6

Methylene Blue

Answer 6

Oxidised: Blue
Reduced: Colourless

Question 7

Triphenyl Tetrazolium Chloride

Answer 7

Oxidised: Colourless
Reduced: Pink

Question 8

Dichlorophenol Indophenol (DCPIP)

Answer 8

Oxidised: Blue
Reduced: Colourless

Question 9

Experiments of Dehydrogenase Activity

Answer 9

•demonstration of H release in a living yeast suspension compared to a control containing boiled yeast

•effect on dehyd activity of adding an intermediate of Krebs cycle (succinate substrate)

•effect of malonate (competitive inhibitor of succinate dehyd) on dehyd activity