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Flashcards in Photosynthesis Deck (16)
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1

What is photosynthesis?

Process by which plants, as well as some bacteria and protists, convert solar energy into chemical energy.

2

What is the chloroplasts most important pigment?

Chlorophyll

3

What are the pigments found in chloroplasts?

Chlorophyll
Carotenoids
Phycobilins

4

Color and structure of pigments found in chloroplasts.

Chlorophyll- green. Porphyrin rings containing mg at center. Includes chlorophyll a, b, c, d.
Insoluble in water

Carotenoids- red, orange, or yellow. Composed of 2 small six-carbon rings connected by a chain of carbons. Insoluble includes carotenes and xanthophylls

Phycobilins- water soluble found in stroma

5

What will be used to separate plant pigments

Chromatography paper

6

The rate at which pigments move friend on what?

Solubility of solvent and degree of attraction to paper

7

Polarity of products:
Chromatography paper
Carotenoids
Organic solvents

Chromatography paper is polar as it has cellulose
Organic solvent is non polar
Carotenoids non polar remain dissolved
Polar pigments get attracted to paper

8

What is retention factor (RF)?

Relates the traveled distance by a pigment to the distance traveled by the solvent during the chromatography process

9

Materials for activity 1: Identification of photosynthetic pigments by partier chromatography

Chromatography paper
Spinach leaf pigments extracted in acetone
Capillary tube
Chromatography solvent of petroleum ether and acetone

10

Formula for retention factor RF

Distance traveled by pigment/distance traveled by solvent

Rd=P/S

11

Materials activity 2a: the action spectrum for photosynthesis

Elodea leaves
Spinach leaves for absorption part
5 percent sodium bicarbonate
Cellophane paper( red yellow green blue violet)

12

How long do you need to wait for oxygen and how many watts?

45 min. 200 watts bulb

13

How many spinach leaves

10-15 in 200 mL

14

How much are you diluting the spinach extract?

9 mL water to 1mL extract

15

What wavelength setting spectrometer?

400 nm increasing 25 nm increments up to 725

16

What does absorption spectrum describe?
Action spectrum?

Different wavelengths of absorbed light use spectrophometer

How effective different wavelengths of light are at initiating photosynthesis. Use different color filters to determine oxygen released.