Plant & animal cells, Bacteria, DNA, Genetic engineering Flashcards

1
Q

What parts do an animal and plant cell have in common?

A

Cell membrane, nucleus, cytoplasm, mitochondria.

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2
Q

What does a plant cell have that an animal cell doesn’t?

A

Cell wall, central vacuole, chloroplasts.

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3
Q

What’s the role of the cell membrane?

A

Controls what enters and leaves the cell, e.g. oxygen, carbon dioxide, glucose.

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4
Q

What is the nucleus and what does it do?

A

It’s a large structure that contains DNA - instructions for the building and working of the cell.

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5
Q

What is cytoplasm and what does it do?

A

It’s a jelly like substance that fills the cell, many chemical reactions take place here.

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6
Q

What’s the role of mitochondria and what are they?

A

They are tiny structures where respiration takes place, releasing energy for cell processes.

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7
Q

What’s the vacuole, where is it found and what is its role?

A

It’s found in a plant cell, it’s full of cell sap which keeps the cells structure rigid.

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8
Q

Name the main parts of a bacteria cell.

A
  • Cell wall
  • Cell membrane
  • Chromosomal DNA
  • Plasmid DNA
  • Flagellum (sometimes)
  • Cytoplasm
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9
Q

What is the difference between chromosomal DNA and plasmid DNA?

A
  • Chromosomal DNA carries most of the bacterial genes
  • Plasmids contain additional genes not found in chromosomes
  • Chromosomal DNA is a single loop
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10
Q

What’s the difference between a light microscope and an electron microscope?

A
  • A light microscope uses light to magnify objects.
  • An electron microscope uses electrons to view an object.
  • An electron microscope is more powerful.
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11
Q

What’s the maximum magnification a light microscope has?

A

x2000

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12
Q

What’s the maximum magnification an electron microscope has?

A

x10 Million

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13
Q

What is adenines base pair?

A

Thymine.

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14
Q

What’s cytosines base pair?

A

Guanine.

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15
Q

What holds the base pairs and DNA strands together?

A

Weak hydrogen bonds.

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16
Q

The nucleus contains______.

A

Chromosomes.

17
Q

Chromosomes contain_______.

A

Strings of genes.

18
Q

What is a gene?

A

A short piece of DNA that codes for a specific protein.

19
Q

Each gene is a length of _____.

A

DNA.

20
Q

What’s the DNA’s structure?

A

Double Helix.

21
Q

How do you extract DNA from a kiwi?

A
  • Mash up the kiwi and mix with salty water and detergent, this breaks open the cells and helps release the DNA from the nuclei.
  • Filter the mixture and add protease enzyme to break up the cells proteins and release more DNA.
  • Ice-cold ethanol is poured into the mixture, this makes the DNA separate from the liquid so it’s easy to lift out.
22
Q

Who used X-Rays to study the structure of DNA?

A

Franklin and Wilkins.

23
Q

Who helped build the double helix model?

A

Watson and Crick by using Franklins photographs as a final clue.

24
Q

How many countries scientists collaborated to decode the human genome?

A

18.

25
Q

Why did it only take a short while for the human genome to be decoded?

A

Many scientists were involved in the project.

26
Q

GE 1: How is the insulin gene cut out of the human chromosome?

A

By using a restriction enzyme.

27
Q

GE 2: What part of the bacteria cell is taken out and how is it used?

A

Plasmid, part of it is cut out using enzymes in order to make room for the insulin gene.

28
Q

GE 3: What happens after the insulin is placed into the plasmid?

A
  • The plasmid is put back into the bacteria.

- The bacteria will reproduce and this time with the human insulin gene.

29
Q

What is 1 advantage of genetic engineering?

A

Any from:

  • Making insulin through bacteria is cheaper and quicker than any other way which means more diabetic people can be treated.
  • Golden rice: Beta-carotene is increased in the rice plant. Beta-carotene makes vitamin A which can prevent illness caused by lack of vitamin A in those who eat mainly rice.
30
Q

What is 1 disadvantage of genetic engineering?

A

Any from:

  • Some diabetic people react badly to this insulin and need a different form.
  • Golden rice seed is more expensive than regular rice so poorer people can’t afford to grow it.