Plasmid Quiz 100! Flashcards
(25 cards)
What gene makes the e Colli glow? It’s the one we added
Bioluminescent jellyfish fluorescent protein GFP
What organism did the glowing gene co,e from
Jellyfish. Aequorea Victoria
What do we do in the lab
Genetic transformation through the insertion of a plasmid
What is the plasmid called
pGLO
What is the ideal organism for transformation? Think, cell amount, reproduction speed, and safety
Bacteria that is non toxic because it is small, and reproduces quickly
SKIP
Skip
Why do we heat up the e Colli before some of the steps
Heat encourages penetration
What was the transformation solution?
CaCl2
What is the purpose of the antibiotic resistance gene in a plasmid?
enables selection of only transformed cells- they break down the antibiotic
What is the purpose of a multiple cloning site in a plasmid?
contains sites for restriction enzymes to insert genes of interest
What is the purpose of a selectable marker in a plasmid?
allows ID of only the bacteria that took up the plasmid on selective media
What is the purpose of the origin of replication in a plasmid?
allows plasmid to replicate in host cells
What is the purpose of plasmid mapping? Select a real world application.
Genetic engineering or gene therapy. Inserting the gene of interest to restore or gain a protein function. LIKE DIABETES MEDS
Why is arabinose do
It activates araC promoter. This initiates the transcription of the GFP gene, causing the protein to be made, and therefore glowing
What does the bla gene do?
This makes Beta-lactamase. This is what breaks down ampicillin, causing antibiotic resistance
Bro what are some reasons your colonies are not growing? :(
Heat shock period was too long, plates were mislabeled and didnt actually have ara, you didnt have enough plasmid, or the incubation was skipped
What could a mutation in a gene do to a protein?
It changes it shape, possibly disrupting the active site. This leads to a loss of OG protein function. An example of this is sickle cell anemia
What part of the central dogma does ara do
Transcription initiation
Tell me all the protein functions due to their shape
Enzymes, transport, structure, storage, hormonal, defense, and contractile
give me 3 different proteins and their general function (category), because we know Mrs Carlson loves active recall
Some correct answers:
Lactase (enzyme) breaks down lactose
Lipase (enzyme)
Structural: keratin (hair and nails), collagen (skin)
Storage: Ferritin (iron)
Hormonal: Insulin
Vectors
Deliver recombinant DNA to an organism ie plasmids, viruses
Ori or and origin of replication
The place so that plasmids know when to start replication
If you insert the DNA of interest in the middle of a beneficial gene it is called
An insertional inactivation because the protein we interrupted wont be complete.
What advantage would there be for an organism to be able to turn on or off particular genes in response to certain conditions?
- switching priority of certain genes (if ur stomach is telling ur body ur hungry, the body is gonna prioritize the hungry feeling in the brain. It wont do this if ur stuffed tho)
- also, antibiotic resistance in the presence of ampicillin. It isnt always making that protein, but in case of antibodies, it will
