Platelet Structure And Estimate

Question 1

Are fragments arise from the megakaryocytes, which is the largest cell found in the bone marrow

Answer 1

Platelets

Question 2

Largest cell in the peripheral blood

Answer 2

Monocytes

Question 3

They are small, moderately refractile bodies that appears as azure granules which scanty light blue cytoplasm when stained

Answer 3

Platelets

Question 4

Platelet undergoes ____, which is a process of nuclear division without cellular division

Answer 4

Endomitosis

Question 5

Platelets are essential for maintaining the balance of bleeding and clotting within the body which is termed as

Answer 5

Hemostasis

Question 6

tube used for platelet count

Answer 6

EDTA

Question 7

What stain is used in platelet count

Answer 7

Giemsa stain

Question 8

Diameter of platelets

Answer 8

2 to 3 um

Question 9

A phenomenon where blood platelets stick together to form clumps, potentially causing a falsely low platelet count in laboratory test

Answer 9

Platelet clumping

Question 10

Platelet clumping results in

Answer 10

Falsely low platelet count

Question 11

A rare in vitro phenomenon where platelets clamp around neutrophils, other leukocytes, or even other cells, giving a rosette like appearance on a blood smear

Answer 11

Platelet satellitism

Question 12

Abnormally large platelets usually greater than seven microns in diameter

Answer 12

Giant platelets

Question 13

This test measures the number of platelets with blood cells and red blood cells.

Answer 13

Platelet count

Question 14

This test can be done by simply preparing a blood smear then it will be evaluated at 100 times magnification

Answer 14

Estimated platelet count

Question 15

The platelet count per ul of blood can be approximated by multiplying the number of platelets seen in 10 microscope fields by ___

Answer 15

15,000

Question 16

Other methods for platelet count

Answer 16

1.Reese-Ecker Method
2.Ammonium Oxalate Technique
3. Direct Method
- Nygard’s
- Guy and Leake’s
- Brenker-Cronkite
- Unopette
4. Indirect Method
- Dameshek
- Fonio’s
- Olef’s

Question 17

Diluting fluid composition of reese-ecker method

Answer 17

•brilliant cresyl blue
•40% formalin
•sodium citrate

Question 18

Procedure of reese-ecker method

Answer 18

1. Draw blood up to 0.5 mark of the rbc pipette
2. Add reese-ecker diluting fluid up to 101 marks
3. Shape the pipette for 1 to 5 minutes
4. Discard 5 to 6 drops and discharge the counting chamber
5. Place the counting chamber on a petri dish with a wet filter paper to prevent evaporation. Let it stand for 10 to 15 minutes to allow the platelet to settle
6. Count the plate that’s in all 25 tertiary squares of central secondary square
7. Compute the platelet count

Question 19

Computation for reese ecker method

Answer 19

Number of cells / mm3 = total number of cells counted/areaxdepthxdilution

Or

Cells counted x 2000

Question 20

Procedure of ammonium oxalate technique

Answer 20

1. Draw blood up to the 1.0 mark of the rbc pipette and ammonia oxalate up to the 101 marks
2. Procedure 2 to six of reese ecker method perform

Question 21

Computation for ammonium oxalate technique

Answer 21

Number of cells/mm3 =total number of cells counted/area x depth x dilution

Or

Cells counted x 1000

Question 22

Direct methods of platelet count

Answer 22

•Nygard’s
•Guy and Leake’s
•Brenker-Conkrite

(NGB)

Question 23

Indirect method of platelet count

Answer 23

•Dameshek
•Fonio’s
•Olef’s

(DFO)

Question 24

The best indirect method of platelet count

Answer 24

Olef’s Method

Question 25

Sources of error of platelet count

Answer 25

•platelet clumps •imperfect sources of blood •blood in edta kept at 20°C