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Physiology I Practical > Practical 1 Haematology > Flashcards

Practical 1 Haematology Flashcards

1
Q

Average blood volume of adult animals

A

60-80 ml/kg bwt

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2
Q

Density of blood

A

1050 g/dm3

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3
Q

relative viscosity of blood

A

3.5

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4
Q

Ph of blood

A

7.35 - 7.45

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5
Q

Osmolarity of blood

A

300 mosmol/l

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6
Q

osmolality of blood

A

300 mosmol/kg

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7
Q

total osmotic pressure of blood

A

700 kPa

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8
Q

oncotic of blood

A

3.5 kPa

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9
Q

freezing point of blood

A

-0.56

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10
Q

hemoglobin concentration of blood

A

120 - 180 g/l

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11
Q

Density of blood plasma

A

1020 g/dm3

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12
Q

Na+ conc of blood plasma

A

140mmol/l

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13
Q

k+ conc of blood plasma

A

4mmol/l

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14
Q

Mg2+ conc blood plasma

A

1.5mmol/l

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15
Q

Ca+ conc mamanal blood plasma total

A

2.5mmol/l

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16
Q

Ca+ conc mamanal blood plasma ionic

A

1.25mmol/l

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17
Q

Ca+ conc poultry blood plasma total

A

5 mmol/l

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18
Q

Ca+ conc poultry blood plasma ionic

A

1.25mmol/l

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19
Q

cl- conc blood plasma

A

110 mmol/l

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20
Q

HCO3- conc blood plasma

A

27 mmol/l

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21
Q

Panorg conc blood plasma

A

2 mmol/l

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22
Q

fibrinogen conc blood plasma

A

3 (1-6) mmol/l

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23
Q

glucose level ruminants blood plasma

A

3 (2-4) mmol/l

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24
Q

glucose level monogastric animals blood plasma

A

5 (3-5.5) mmol/l

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25
Q

glucose level poultry blood plasma

A

10 (9-11) mmol/l

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26
Q

cholesterol level blood plasma

A

5 (1 - 8) mmol/l

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27
Q

residual nitrogen level blood plasma

A

15-25 mmol/l

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28
Q

urea blood plasma

A

3-10 mmol/l

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29
Q

creatinin blood plasma

A

50 - 200 umol/l

30
Q

Hematocrit in venous blood average

A

0.35-0.45 l/l
35% - 45%

31
Q

blood clotting time horse

A

20-25 min

32
Q

blood clot time cattle

A

5-15min

33
Q

blood clot time swine

A

5-6 min

34
Q

blood clot time dog

A

6-7 min

35
Q

blood clot time cat

A

7-9

36
Q

Erythrocyte sedimentation rate horse

A

60- 90 mm/h

37
Q

Erythrocyte sedimentation rate cattle

A

0-2 mm/h

38
Q

Erythrocyte sedimentation rate swine

A

1-14mm/h

39
Q

Erythrocyte sedimentation rate dog

A

5-10 mm/h

40
Q

Erythrocyte sedimentation rate cat

A

5-10 mm/h

41
Q

lymphocytic species: cattle, swine, sheep, domestic fowl
Lymphocyten

A

60 (55-75) %

42
Q

lymphocytic species: cattle, swine, sheep, domestic fowl Neutrophil granulocyte

A

35 (15-45) %

43
Q

lymphocytic species: cattle, swine, sheep, domestic fowl Eosinophil granulocyte

A

0-5

44
Q

lymphocytic species: cattle, swine, sheep, domestic fowl basophil granulocyte

A

1

45
Q

lymphocytic species: cattle, swine, sheep, domestic fowl Monocyte

A

1-10

46
Q

neutrophilic species: horse, dog, cat Lymphocyte

A

35 (15-45)%

47
Q

neutrophilic species: horse, dog, cat NEUTROPHIL GRANULOCYTE

A

60 (55-75) %

48
Q

neutrophilic species: horse, dog, cat EOSINOPHIL GRANULOCYTE

A

0-8

49
Q

neutrophilic species: horse, dog, cat BASOPHIL GRANULOCYTE

A

1

50
Q

neutrophilic species: horse, dog, cat MONOCYTE

A

0-7 %

51
Q

Protein conc of body fluids Mammals, blood plasma

A

60-80 g/l;
albumin 35-50 %, globulins 50-65 %

52
Q

Protein conc of body fluids poultry, blood plasma

A

30-40 g/l
albumin 35-50 %, globulins 50-65 %

53
Q

protein conc body fluids interstitial fluids

A

30-40g/l

54
Q

protein conc of body fluid lymph

A

30-40g/l

55
Q

protein conc of body fluids cerebrospinal fluid

A

0.25 - 0.4 g/l

56
Q

Effects of heparin?

A

prevents blood from coagulating by catalysing the thrombin-antithrombin reaction

57
Q

What is the function of methanol in fixation?

A

fix the smear and precipitate the proteins

58
Q

Buffy coat, explain

A

White blood cells have lower density than red, and will form thin layer on the top after centrifugation

59
Q

How to obtain blood serum

A

Centrifuge blood sample where fibrinogen is changed into fibrin by shaking in flask containing small glass beads

60
Q

Defirbrinated blood

A

Shake flask with blood and small glass beads to change fibrinogen into fibrin

61
Q

What happens with the hematocrit level while increasing the epinephrine level

A

Increase

62
Q

Anticoagulants, give examples.

A

sodium citrate and Ethylene Diamine Tetraacetic Acid (EDTA)

63
Q

Lee white method

A

Tilt blood in tube in intervals in 3 minutes to see if it coagulates

64
Q

Def. of hematocrit

A

Ratio of red blood cells to the total blood volume

65
Q

Venous hematocrit larger than arterial, explain.

A

Because of ion transport and water flux associated with the CO2 transport

66
Q

Increased erythrocyte sedimentation rate

A

By displacing the albumin the surface charge decrease

67
Q

By displacing the albumin the surface charge decrease

A

nucleus: beanshape (young), elongated (middleage), segmented (mature), light pink cytoplasm, different granules, 10 μm diameter, enzymes: lysozyme, myeloperoxidase)

68
Q

Describe the hetrophil granulocytes (birds)

A

Nucleus: seveeral segments, pink cytoplasm, pale red granules

69
Q

Describe the eosinophil granulocytes

A

Nucleus: segmented (usually bi-lobed), large bright red granules, 14 μm diameter, Enzmes: histaminase, acid & alkaine phosphatase, eosinphil basic & cationic protein)

70
Q

Describe the basophil granulocytes

A

nucleus: hardly segmented, large blue granules, 15 μm diameter, Enzymes: protease

Physiology I Practical (5 decks)

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