Practical 1: Investigate the effect of caffeine on heart rate in Daphnia

Question 1

Daphnia practical.

Answer 1

-Dilute the caffeine solution with distilled water to produce several different concentrations.
-Use cotton wool to restrict movement and place a Daphnia in the solution, on the cover slide.
- Use a stop clock to time a minute and record the number of heartbeats.
- Repeat the experiment, replacing the distilled water with a caffeine solution.
- Repeat each concentration to calculate a mean.

Risks- contammination, broken glass

variables- control = species
- independent = caffiene solution - dependent = bpm of daphnia

Plot on graph