Practical Skill: Investing Factors Affecting Enzyme Reaction Rates

Question 1

Limitations

Answer 1

The above method can be adapted to control temperature by using a water bath at 35℃
All solutions that need to be used (starch, amylase, pH buffers) should be placed in a water bath and allowed to reach the temperature (using a thermometer to check) before being used

A colorimeter can be used to measure the progress of the reaction more accurately; with a solution containing starch being darker and glucose lighter (as a result of the colour-change of iodine) – this will affect the absorbance or transmission of light in a colorimeter

Question 2

You can test for bile affecting enzyme rate of reaction by using

Answer 2

different bile salt concentrations