Practice Set 6 Flashcards
(28 cards)
Which of the following covalent modifications is not true?
H1 methylations are very important in opening chromatin.
Which of the following activities is not part of the chromatin remodeling mechanism?
Oxidation to H4.
Which of the following methods is used to analyze open chromatin structure?
DNA hypersensitivity with nuclease treatment.
Which of the following methods is used to detect genome-wide protein distribution on chromosome
regions?
ChIP.
In Drosophila, heterochromatin
All are correct
a. is condensed throughout the cell cycle.
b. contains very small number genes.
c. is located near the centromeres.
Epigenetics could cause gene silencing through
All are correct
a. DNA methylation
b. histone modification
c. non-coding RNA, e.g., miRNA, siRNA
Position effect variation
All are correct
a. is an epigenetic phenomenon.
b. involves no DNA sequence change
c. was shown originally by using a chromosome inversion
Which of the following describes the histones associated with the nucleosomes of active genes?
Rich in acetyl groups (i.e., hyperacetylated)
Compared to heterochromatin, euchromatin is
rich in genes and comprised of loosely packed nucleosomes.
The phenomenon in D. melanogaster known as Position-Effect Variegation (PEV) provides powerful evidence that:
chromatin structure is able to regulate gene expression.
In mice, the igf2 allele is only expressed if
it is inherited from the father.
Regions of a chromosome that are bundled in highly condensed chromatin are known as
heterochromatin.
A Barr body refers to
None are correct
a. a sex-linked trait in cats.
b. a recessive trait in fruit flies.
c. an inactivated Y chromosome.
d. spore formation in Neurospora.
“Traditional” Whole Genome Sequencing (WGS) employs which of the following techniques?
All are correct
a. The construction of genomic libraries
b. Cloning of DNA in bacterial systems
c. Sanger dideoxy DNA sequencing
Sanger DNA sequencing determines the nucleotide sequence because:
dideoxy nucleotides terminate the growing DNA strand.
Pyrosequencing determines the nucleotide sequence because:
PPi is released from the growing DNA strand.
The TRANSCRIPTOME is defined as:
the sequence and expression patterns of all transcripts.
Regarding genomic imprinting, which of the following statements is NOT true
It is an inheritance process of the classical Mendelian inheritance
In mice the Igf-2 gene is maternally imprinted. To generate parthenogenic mice that inherited both Igf-2 alleles maternally, a genetic-engineering experiment was carried to
allow only one of the maternally derived alleles to express
The phenotypical difference between the XX and XO genotypes can be best explained by
the XO genotype only has a single dose of genes located in two pseudoautosomal regions
Regarding genomic imprinting, which of the following statements is NOT true
It is an inheritance process of the classical Mendelian inheritance
In mice the Igf-2 gene is maternally imprinted. To generate parthenogenic mice that inherited both Igf-2 alleles maternally, a genetic-engineering experiment was carried to
allow only one of the maternally derived alleles to express
Which of the following covalent modifications is NOT true?
H1 methylations are very important in opening chromatin.
Which of the following activities is not part of the chromatin remodeling mechanism?
Oxidation to H4.
