Practicum

Question 1

Antibodies that may cause a HDN

Answer 1

ABO, Rh, Kell, Duffy

Question 2

Antibodies that may cause delayed HTR

Answer 2

Kidd

Question 3

If R2R2 patient has a 3+ reaction and is compatible with R1r donor what Ab is present?

Answer 3

anti- E would be present

Question 4

How are cold agglutinates and rouleaux excluded from testing?

Answer 4

Cold agglut- absorption

Rouleaux- saline replacement

Question 5

List the antibodies detected at different phases.

Answer 5

AHG- Kell, Duffy, Kidd
IS- Lewis, MN,P1
37- Rh, MN

Question 6

Interval between donations

Answer 6

8 weeks

Question 7

minimum Hgb

Answer 7

minimum Hgb greater than or equal to 12.5 g/dL

Question 8

minimum Hct

Answer 8

greater than or equal to 38%

Question 9

Maximum total volume of blood drawn

Answer 9

525 mL max (normally 450 mL)

Question 10

Minimum Body Weight

Answer 10

greater than or equal to 110 lbs (50kg)

Question 11

Maximum Body Temperature

Answer 11

37.5 C/ 99.5 F

Question 12

How long is the deferred period if person spent time in a malaria area?

Answer 12

1 year

Question 13

Mother- R1r
child1- rr
child2- R2r
What would the father be?

Answer 13

R2r

Question 14

What blood type is used for emergency release and alternate blood selection?

Answer 14

O negative blood

Question 15

Refrigerator temperature

Answer 15

1-6 C

Question 16

Length of time recipient samples are saved

Answer 16

7 days

Question 17

Shelf life after breakage of hermetic seal

Answer 17

24 hours

Question 18

Temperature range during whole blood shipment

Answer 18

1-10 C

Question 19

Reason to discard a unit of blood

Answer 19

unit looks hemolyzed

Question 20

Which blood group has the most reactivity with anti-H?

Answer 20

Group O is the strongest

Question 21

Hemophiliac patient would need what type of blood component?

Answer 21

Cryoprecipitate

Question 22

What is unique about the Lewis system?

Answer 22

The only blood group system not manufactured by the RBCs. Pregnant women may present as Le(a-b-) and produce Lewis antibody even though true phenotype is Le(a-b+). Can show up in saliva and tears.

Question 23

What antibodies are found in Bombay individuals?

Answer 23

All of them (Anti-A, -B, -AB, -H)

Question 24

What blood group antigens are enhanced or destroyed by enzymes?

Answer 24

Enhanced: Lewis, Rh, Kidd
Destroyed: Fy(a), Fy(b) (»Duffy Inhibited) MNS

Question 25

In the panel given remember to choose the answer with...

Answer 25

2 negative and 1 positive for both antibodies

Question 26

What would you expect if a Rh neg patient who has never been transfused or pregnant was transfused with Rh pos donor blood?

Answer 26

No transfusion reaction because no antibodies are present because no prior exposure

Question 27

When should RhoGAM be given?

Answer 27

Rh negative mother with Rh positive baby

Question 28

Elution

Answer 28

To determine allo or auto ab, To determine HDN Used to remove antibodies bound to sensitized RBCs

Question 29

Forward grouping

Answer 29

Using forward known serum antibodies to detect unknown antigens

Question 30

DAT

Answer 30

if POSITIVE perform elution to remove antibody to ID specific eluate used to diagnose HDN, HTR & AIHA also, in ((( Direct Coombs use patient cells!!!))))

Question 31

Polyspecific AHG

Answer 31

Has both anti-complement/ IgG

Question 32

What is Anti- A1 lectin used to detect?

Answer 32

used with A1- ABO discrepancy

Question 33

Wharton's jelly

Answer 33

on cord blood

Question 34

Why aren't reverse blood groupings done on newborns?

Answer 34

Antibodies are not yet developed

Question 35

Define febrile nonhemolytic reaction

Answer 35

chills, fever, increase in temperature by 2 C

Question 36

What could a weak subgroup of A cause?

Answer 36

ABO discrepancy

Question 37

What would you use to distinguish between an A1/A2 discrepancy?

Answer 37

Dolichus Biflorus

Question 38

If the mother is O and the baby is A and there is HDN what would you transfuse with?

Answer 38

Group O Blood

Question 39

Would A2B with anti- A1 cause a discrepancy?

Answer 39

YES

Question 40

What would the next step be to identify the cause of a strong positive DAT in cord blood and mother sample?

Answer 40

preform an elution on the sample

Question 41

Why are the cells washed in the AHG test?

Answer 41

to prevent neutralization by globulins

Question 42

What is the reason for a mixed- field result on a DAT for a patient who has been previously transfused?

Answer 42

2 cell populations in the patients blood

Question 43

What type of sample do you need for compatibility testing?

Answer 43

FRESH serum

Question 44

Why do you need a FRESH serum specimen?

Answer 44

To preserve complement

Question 45

What type of elution is required for HDN

Answer 45

Acid Elution

Question 46

Deferral

Answer 46

If they tested positive for HBsAg