preparation of RCS, serial dilution

Question 1

commonly used reagent for many serological procedures

Answer 1

RCS

Question 2

RCS is usually prepared from anticoagulated whole blood collected in __, __, or __ tubes

Answer 2

citrate, oxalate, EDTA

Question 3

washed red cells are suspended in a specific volume of __ saline to obtain the desired concentration of the final suspension

Answer 3

isotonic

Question 4

in serologic assays, __(%) rRCS are utilized as indicators of antigen-antibody reactions in vitro

Answer 4

2-5%

Question 5

t/f: ratio of antibodies in a serum to antigens in a RCS may dramatically affect the sensitivity of serologic assays

Answer 5

t

Question 6

washing is donr __ times

Answer 6

3

Question 7

any yellow appearance in the final wash indicates __ and suggests that __ are still present

Answer 7

incomplete washing, traces of plasma

Question 8

if the supernatant fluid turns red, __ has occurred

Answer 8

hemolysis

Question 9

technique commonly employed in several assays in immunology and serology

Answer 9

serial dilution

Question 10

popular type of series dilution wherein each successive tube will have half the amount of the original concentrated solution

Answer 10

doubling / two-fold dilution

Question 11

__ can be used to identify and distinguish a number of substances, such as foreign agents or pathogens

Answer 11

antibodies

Question 12

cardinal features of an antigen-antibody reaction are __, __, and __

Answer 12

precise, reversible, invisible

Question 13

t/f: in vivo demonstrations of antigen-antibody reactions are vital in clinical applications, assessments of the immune response, and research

Answer 13

f (in vitro)

Question 14

two of the most common endpoints used in the laboratory detection of antigen-antibody reactions in vitro

Answer 14

aggregation of cells, hemolysis