Presentation 6: Combination Therapy of Zing and Trimethoprim Inhibits Infection of Influenza A in chick embryo Flashcards
Explain the Influenza A genome
8 negative sense ssRNA segments
Each segment encodes for a protein that has specific function
RdRP is important for transcribing and replicating the viral genome
What family is Influenza A part of?
Orthomyxoviridae
What was the question in this paper?
Can TMP and Zinc acting on the host reduce the lethal effect of influenza A virus in chick embryo model
What is the hypothesis of this study?
The correct ratio of TMP and zinc may treat respiratory RNA virus infections
Why was TMP and Zinc used?
Zinc ions are found to inhibit RSV
TMP inhibits folate dehydrogenase affecting nuclear DNA
Why was a chick embryo used?
More resistant to zinc toxicity
Needs zinc for normal development
Easy culture medium and low cost
How were viruses identified in this study?
Positive swabs and fluorescence immune assay and PCR to detect influenza
How was virus isolated and detected?
Experimented repeated three times
To create viral stock allantoic fluid was collected from the last experiment
A hemagglutination test was done
How was EID50 determined?
12 eggs per dilution
Experiment repeated 3 times
Dilution that caused 50% death of embryos
How was the effect of Tri-Z determined?
Embryo survival
What was discovered from this study?
1) Embryos treated with a combination of trimethoprim and zinc at an optimal ratio reduces the lethal effect of influenza A virus regardless of viral dose
2) Embryos treated with TMP, zinc, saline separately had no effect on survival, embryos did not survive
3) Embryos treated with convalescent serum, embryos survived
What happened with the HA inhibition test?
At ratio 0.18 Zn to TMP inhibition is possible
Zn and TMP separately did not produce an observable HAI effect
