Pretransfusion Testing Flashcards

1
Q

goal of pretransfusion testing

A

to ensure compatibility between donor product and recipient

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2
Q

who does the donor testing

A

Canadian Blood Services

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3
Q

recipient testing

A

performed by hospital transfusion service

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4
Q

this ensures compatibility between donor cells and patient plasma (major)

A

crossmatch

  • ABO
  • all products must be ABO compatible
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5
Q

step after crossmatch

A

allocation/ tagging

  • allocate to one patient only
  • unit is tagged (first, last, ULI, BBIN)
  • designated for patient
  • 96 hrs
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6
Q

issuing

A
  • after allocation

- leaves blood blank

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7
Q

pretransfusion testing criteria

A
  • informed consent
  • patient ID, sample collection, labeling
  • patient history
  • ABO Rh
  • antibody screen
  • crossmatch
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8
Q

T or F. ABO Rh is done with every single pretransfusion testing

A

T

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9
Q

when identifying patients for pretransfusion testing …

A
  • two means of identifying = nurse and phlebotomist OR barcode reader and phlebotomist
  • unique identifier, unique lifetime identifier, PHN, military ID, RCMP ID
  • legal first and last name
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10
Q

specimen type for pretransfusion

A

2 EDTA

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11
Q

what information MUST be on the requisition?

A
  • first and last name
  • ULI
  • BBIN/TSIN
  • time and date
  • 2 signatures (nurse and collector or barcode)
  • patient ID + labeling MUST occur at bedside*
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12
Q

what info must be on the specimen tube?

A

first and last name
ULI
BBIN

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13
Q

BBIN

A

blood bank identification number

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14
Q

TSIN

A

transfusion service identification number

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15
Q

what must you do if there are any missing inf, illegible, or mismatches (letter/number)?

A
  • reject specimen and request recollection

- fill out occurrence management form (RLS)

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16
Q

when are full clerical checks performed?

A
  • receipt in lab (acceptance/rejection)
  • ABO, Rh, Ab screen
  • result entry
  • constant checks (accession) performed continuously throughout testing
17
Q

T or F. We check transfusion records on each patient with every collection

A
T!
- ABO Rh type
- antibody screen results
- antibody identity/Ag typing
- adverse events (transfusion rxns)
- previous transfusion/pregnancy
- special instructions/products
 > irradiated, washed, IgA deficient
18
Q

how many ABO Rh tests must be performed to comply with electronic XM?

A

2

18
Q

how many ABO Rh tests must be performed to comply with electronic XM?

A

2
- historical type is OK if performed by testing lab
- ABO Rh is always done on current sample
- any discrepancy must be resolved
- mismatch from historical record must be investigated
> repeat patient ID, collection

19
Q

what is an anitbody screen?

A
  • detects Abs to clinically significant antibodies

> Rh, Kell, Duffy, Kidd, Ss

20
Q

minor crossmatch

A

“minor” crossmatch is designed to test opposite compatibility: The donor’s serum/plasma with the recipient’s red cells. For example, to perform a minor crossmatch between a group A blood donor and a group B recipient, blood bank personnel would mix the donor’s plasma/serum (containing anti-B antibodies) with the recipient’s red blood cells

21
Q

what happens after crossmatch?

A
  • allocation or tagging
  • issuing
  • traansfusion
22
Q

allocation

A
  • unit is tagged with first name, last name, ULI, BBIN
  • designated for THAT patient
  • 96 hrs
23
Q

what does issuing mean?

A

when the allocated unit leaves blood bank

24
Q

process of transfusion

A
  • two nurses ID patient (vs tag)
  • transfusion started within thirty minutes of issue
  • unit is transfused within 4 hrs
  • vital signs taken before, 15 min in, and after infusion of product
25
Q

advantages of a type & screen

A
  • prevents allocation of blood that won’t be needed
  • permits more stock to be available
  • makes good use of time and resources
  • decreases cost
  • if blood IS required, electronic XM is quick!
25
Q

advantages of a type & screen

A
  • prevents allocation of blood that won’t be needed
  • permits more stock to be available
  • makes good use of time and resources
  • decreases cost
  • if blood IS required, electronic XM is quick!
26
Q

type and screen

A
  • patent ID, valid sample
  • history check
  • ABO Rh
  • Antibody screen; if no history and antibody screen is neg = STOP
  • then when need transfusion = electronic XM
27
Q

how does the procedure for antiboy screen differ if there is a history of clinically significant Abs or antibody screen is positive

A
  • antibody identification, DAT
  • antigen type patient (should be Ag neg)
  • antigen type donors (based on Ag frequency)
  • serologically crossmatch 2 units
28
Q

issuing blood in an emergency

A
  • if ABO unknown or unresolved discrepancy = issue group O
  • if Rh unknown discrepant =
    > issue Rh pos to males > 4 mos and females >45 y/o
    > issue Rh neg to males < or = 4 mos nd females < or = 45 y/o
  • all unmatched blood is k neg in EDM zone *
29
Q

limitations of pretransfusion testing: antibody screen

A

may miss Abs to low frequency antigens not represented on screen cells

30
Q

limitations of pretransfusion testing: IS crossmatch

A
  • false neg due to weak ABO Ab - important to check ABO typing
  • false pos due to cold IgM antibody reacting at RT (allo or auto)
31
Q

limitations of pretransfusion testing: serological XM

A
  • weak Ab and dosage (donor cell heterozygous expression of Ag)
  • donor Ag neg for corresponding Ab
  • screening cells are more sensitive than donor cells