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MCAT Biochem > Protein Analysis > Flashcards

Flashcards in Protein Analysis Deck (5)
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X-ray crystallography

protein structure is determined by this after the protein is isolated


Amino acid composition can determined by simple hydrolysis, but amino acid sequencing require sequential degradation, such as the.. Edman degradation

Edman degradation


protein concentration is determined colorimetrically by:

UV spectroscopy or through a color change reaction


3 tests that tests for protein and have different advantages and disadvantages:

BCA assay
Lowry reagent assay
Bradford protein assay


Most common test to use to test for protein

Bradford protein assay