Protein Processing

Question 1

What antibiotic inhibits Initiation in Translation?

Answer 1

Streptomycin (Prok)

Question 2

Which antib’s inhibit elongation in translation? for E and P

Answer 2

Euks:
Shiga toxin
Ricin
Cycloeheximide
Diphtheria toxin
Prok:
Tetracyline
Clindamycin
Erthromycin
Both: Puromycin
Chloramphenicol(p and mito)

Question 3

Tetracyclin

Answer 3

Elongation
30s
blocks entry of aminoacyl-tRNA

Question 4

Streptomycin

Answer 4

30s
Initiation
- binding fMet-tRNA, 30s can’t bind to 50s

Question 5

Shiga Toxin and Ricin

Answer 5

60s

- entry of aminoacyl-tRNA

Question 6

Clindamycin and Erythromycin

Answer 6

50s

Elongation/Translocation of the ribosome

Question 7

Diphtheria Toxin

Answer 7

Elongation/Translocation

– GTP bound EF2-GTP

Question 8

Chloramphenicol

Answer 8

Elongation (P and mitos)

– peptidyl transferase

Question 9

Cycloheximide

Answer 9

Elongation (euks)

–peptidyl transferase and -peptide bond formation

Question 10

Puromycin

Answer 10

Elongation in both E&P

Premature chain termination by resembling the 3’ end of aminoacylated tRNA

Question 11

Chaperonins

Answer 11

(HSP60) heat shock protein w/ barrel-shaped components that admit unfolded proteins and catalyze their folding via ATP