Protein synthesis Flashcards
(28 cards)
how many combinations of codons
64
code written in what direction
5’-3’
AUG
initiation codon (meth)
UAA,UGA,UAG
stop codons
which 2 AA have only one codon
meth and trp
mitochondria has how many proteins that it can synthesize itself
13
what sequence is added after transcription to tRNA
CCA on 3’
which side is CCA added to
3’
describe aminoacylation of tRNA
o on carboxyl side of AA attacks the alpha phosphate in ATP and is now activated, class 1 or class 2 aminoacyl-tRNA synthase can now help tRNA to bond
how do class 1 and class 2 aminoacyl- tRNA synthases the
class one attacks 2’ OH whereas class 2 attacks 3’
fMet is the first codon for peptide chains in the ____
mitochondria
eIF
eukaryote initiation factors
which eIF brings in tRNA (met)
eIF 5B attached to GTP
where does tRNA met bind to
P site
is mRNA present when tRNA (met) binds?
no
what happens when mRNA comes into pre-initiation complex
- requires energy ATP
- gets scanned untill first AUG is found (the ones before dont get decoded
what happens afte AUG is found
eIF leave and large subunit (60S) comes in forming the 80S ribosome
which elongation factor helps form the peptide bond?
EF-p
which elongation factor binds tRNA into A site?
EF-tu with GTP
which elongation factor pushes dipeptide from A site to P site?
EF-G with GTP
what comes in to inititate termnation
release factor - hydrolyzes the peptidyl link and components dissociate
which terminal would the post translation modification of acetylation aoccur?
the N-terminal of an amino acid
what are other examples of post translational modifications
removal of the Met or fmet
removal of some specific segments in the chain
attaching carbohydrates
E1
activating (ATP)
