Proteins

Question 1

What is the reference value for BUN?

Answer 1

8-23mg/dL (2.9-8.2 mmol/L)

Question 2

It is the major end product of protein and amino acid catabolism.

Answer 2

Blood Urea Nitrogen (BUN)

Question 3

IDMS stands for?

Answer 3

Isotope Dilution Mass Spectrometry

Question 4

Also known as direct method.

Answer 4

Chemical method

Question 5

What is the normal value of creatinine for males?

Answer 5

0.9-1.3 mg/dL (80-115 umol/L)

Question 6

What is the normal value of creatinine for females?

Answer 6

0.6-1.1 mg/dL (53-97 umol/L)

Question 7

End product of muscle metabolism.

Answer 7

Creatinine

Question 8

Give one muscular disease that elevate creatinine levels

Answer 8

Muscular dystrophy, poliomyelitis, hyperthyroidism, or trauma

Question 9

Creatinine is used to monitor this function

Answer 9

Renal function

Question 10

It is the reference method in creatinine determination.

Answer 10

IDMS

Question 11

Kjeldahl Method detects the nitrogen present in the ammonia.

Answer 11

TRUE

Question 12

Lowry Method Principle: Oxidation of phenolic compounds such as tyrosine and tryptophan reduces the PT-PMA reagent (folin-ciocalteu) to give a green color.

Answer 12

FALSE (DEEP BLUE COLOR)

Question 13

Creatinine is not reused in the body’s metabolism, solely as a waste product.

Answer 13

TRUE

Question 14

BUN is freely filtered at the glomerulus but is reabsorbed substantially in the distal convoluted tubule and inner medullary collecting duct.

Answer 14

FALSE (Correct answer: PROXIMAL CONVOLUTED TUBULE)

Question 15

BUN is a good indicator of oxygen intake and the state of hydration.

Answer 15

FALSE (Correct answer: NITROGEN)

Question 16

Turbidimetry is based on the measurement of scattered light by a solution containing fine particles.

Answer 16

FALSE (Correct answer: NEPHELOMETRY)

Question 17

In salt fractionation test, globulins are separated from albumin by a precipitation process using sodium salt.

Answer 17

TRUE

Question 18

Dye-binding method has the highest analytical sensitivity.

Answer 18

FALSE (Correct answer: FOLIN-CIOCAL TEU OR LOWRY METHOD)

Question 19

Ultraviolet Absorption Method Principle: Migration of charge particles in an electrical field.

Answer 19

FALSE (Correct answer: SERUM PROTEIN ELECTROPHORESIS)

Question 20

Kjeldahl Method is the most widely used method, recommended by the International Federation of Clinical Chemistry (IFCC) expert panel.

Answer 20

FALSE (Correct answer: BIURET METHOD)

Question 21

Hepatic disease (BUN)

Answer 21

Decreased

Question 22

Pregnancy (BUN)

Answer 22

Decreased

Question 23

Chronic renal disease (BUN)

Answer 23

Increased

Question 24

Congestive heart failure (Creatinine)

Answer 24

Increased

Question 25

Decreased muscle mass (Creatinine)

Answer 25

Decreased

Question 26

Condition associated with the decrease in Alpha 1 globulin.

Answer 26

ALPHA 1 ANTITRYPSIN DEFICIENCY

Question 27

What are the ions that are bound to albumin?

Answer 27

CALCIUM, MAGNESIUM

Question 28

What band does C reactive protein belong to?

Answer 28

GAMMA BAND

Question 29

Condition in which there is a beta-gamma bridge formed in the electrophoresis pattern.

Answer 29

LIVER CIRRHOSIS

Question 30

Condition where in there is an increase in all of the bands.

Answer 30

INFLAMMATION

Question 31

The most common stain used in serum protein electrophoresis?

Answer 31

COOMASSIE BRILLIANT BLUE

Question 32

What is the pH of zonal electrophoresis?

Answer 32

8.6 pH

Question 33

A stain that can detect small amounts of proteins in a gel and is highly sensitive and selective.

Answer 33

AMIDO BLACK

Question 34

It is identified and quantified optically by change in refractive index at the boundaries among protein bands.

Answer 34

MOVING BOUNDARY OR FRONTAL ELECTROPHORESIS

Question 35

Used as an anticonvection solid support medium in zonal electrophoresis.

Answer 35

FILTER PAPER

Question 36

Two most common protein found in the blood

Answer 36

ALBUMIN AND GLOBULIN

Question 37

A condition that is characterized by a buildup of abnormal amyloid deposits in the body. This happen when total protein level increases

Answer 37

AMYLOIDOISIS

Question 38

Severely decreased TPAG commonly related that patient has?

Answer 38

LIVER CIRRHOSIS

Question 39

It consist of more than 50% of the total serum proteins and also maintains oncotic pressure and transports hormones, vitamins and drugs throughout the body.

Answer 39

ALBUMIN

Question 40

Primary purpose of TPAG test?

Answer 40

ASSESS/CHECK THE LEVELS OF VARIOUS PROTEINS OF VARIOUS PROTEINS IN THE BLOOD AND IF THE LIVER AND KIDNEY IS FUNCTIONING PROPERTY

Question 41

It is the final product of catabolism of purine and nucleic acids.

Answer 41

URIC ACID

Question 42

How many percent or uric acid is being eliminated by renal excretion?

Answer 42

70%

Question 43

Kidney disease and/or kidney stones may be caused if uric acid levels are?

Answer 43

ELEVATED/INCREASED

Question 44

Reference Method for the analysis of uric acid?

Answer 44

ISOTOPE DILUTION MASS SPECTROMETRY

Question 45

What is the required specimen for uric acid? Give one.

Answer 45

HEPARINIZED PLASMA, SERUM, OR URINE

Question 46

What is the technique that separates proteins based on their movement in an electric field?

Answer 46

SERUM PROTEIN ELECTROPHORESIS

Question 47

What does gel matrix usually contain?

Answer 47

AGAROSE OR POLYACRYLAMIDE

Question 48

It provides a medium through which proteins can migrate based on their size and charge, allowing for their separation and analysis.

Answer 48

GEL MATRIX

Question 49

In what charge of the electrode does positive charge migrate?

Answer 49

NEGATIVE (cathode)

Question 49

The migration of proteins is based on what?

Answer 49

SIZE AND SHAPE