Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Clinical Chemistry > Proteins > Flashcards

Proteins Flashcards

(54 cards)

Start Studying
1
Q

Unit For PROTEIN

Unit For Carbs and Lipid

A

g/dL

mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Proteins Negatively Charged

Positively Charged

A

Alkaline pH

Acidic pH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Also known as Amide Linkages; hold amino acids together

A

Peptide bonds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

8 Essential amino acids

A 
Isoleucine
Leucine
Lysine
Threonine
Tryptophan
Phenylalanine
Methionine
Valine
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Quaternary structure:

2 subunits ex.

4 subunits ex

A

Creatine kinase

LDH, Hemoglobin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Proteins affected by pH, ionic strength, temp, dielectric constant

A

Differential solubility

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Can be accomp thru Ultracentrifugation or dialysis

A

Molecular size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Can be accomp thru Mass Spectro

A

Molecular mass

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Done thru Chromatography

A

Surface adsoption

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Ion exchange chroma

Electrophoresis

A

Electrical Charge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Protein digestion starts?

Completed?

A

Stomach

Small Int.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Responsible for digesting proteins in stomach

A

Pepsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

6 Major Plasma Protein Fractions

A 
Prealbumin
Albumin
A1 glob
A2 glob
Beta glob
Gamma glob
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Gc globulin

A

Alpha 1 globulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

3 alpha 2 globulins

A

Ceruloplasmin
Haptoglobin
A2 Macroglobulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

CRP

Immunoglob are examples of?

A

Gamma globulins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Also known as transthyretin

Faster in migration than Albumin

2nd most predominant protein in CSF

A

PREALBUMIN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Carrier protein for thyroid Hormones and Vitamin A

A

Prealbumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Normal life span of albumin

A

15-19 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Decreases during Inflam

Negative Acute Phase reactant

A

Albumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Dehydration = to ____ serum level of albumin?

A

High albumin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q 
Edema
Burns
Ascites
Hepatic dse
Level of Albumin?
Study These Flashcards
A

Low Albumin levels

23
Q

Carrier of Steroid hormones

Study These Flashcards
A

A1 acid glycoprotein

24
Q

Decrease AFP Dses

Study These Flashcards
A

Down syndrome

Trisomy 18

25
High AFP levels dses?
Germ cell tumors | Hepatocellular carcinoma
26
Account to 90 % beta globulins? Also known as Siderophilin
Transferrin
27
Soluble storage of Fe
Ferritin
28
Transports Free Hb Transports Heme
Haptoglobin Hemopexin
29
An oxidase that decreases in Wilsons Dse
Ceruloplasmin
30
Protein present in Plasma
Fibrinogen
31
Method used to measure Fibrinogen, uses Ammonium sulfate Sodium chloride
Parfentjev Method
32
Crp found on what region during electrophoresis??
Beta Some books gamma
33
Bence jones increase in what dse?
Multiple Myeloma
34
Protein that dissolves at 100 C Precipitates at?
Bence Jones Protein 40-60 C
35
Heme containing Protein on Striated skeletal muscles and Cardiac
Myoglobin
36
Troponin 3 subunits
Inhibitory Tripomyosin binding Calcium
37
Spx for protein
Serum/24 hr Urine/ serous fluids NO lipemia, Hemolysis
38
Based on digestion of protein, measure nitrogen
Kjeldahl method
39
Steps of Kjeldahl method
Initial precipitation Acid digestion Quantification of Ammonium ions
40
How are ammonium ions quantified in Kjeldhal method??
Alkalinization Distillation Acid Titration Nesslerization
41
ALK-Distillation-Titration done using ?
Alkali Boric Acid HCL
42
Nesslerization done using
HgI2 | KI
43
Reagents in Buiret Method? RANK
Rochelle Salt Alkaline CuS04 NaOH KI
44
Also known as Rochelle salt Used to complex cupric ion Prevent Precipitation of cupric ions
Sodium potassium tartrate
45
Based in formation of VIOLET colored chelate between Cu ions
Biuret Method
46
Spx is mixed with Alkaline Copper Solution ff by Folin Ciocaltue rgnt
Lowry Method
47
Used for urine and CSF Protein determinations
Salt precipitation Test (Turbudity)
48
Ppts globulins than albumin
TCA acid
49
Seen in Px with Liver cirrhosis Inc IgA in beta and gamma region
Beta Gamma bridging effect
50
Seen in cases of monoclonal gammopathy
Gamma spike
51
Seen in nephrotic syndrome
A2 macroglobulin elevation Albumin decrease
52
Emphysema
A1 antitrypsin deficiency
53
Reference. range Total protein=
6.5- 8.3 g/dL
54
Albumin range
3.5 - 5.5 g/dL

Clinical Chemistry (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions