Proteome

Question 1

An important tool in protein purification is an assay for the protein of interest. T/F

Answer 1

True

Question 2

Protein purification allows the reductionistic study of the isolated protein. T/F

Answer 2

True. Purification allows for the identification of different proteins due to wavelength.

Question 3

Separation techniques are needed in order to accomplish protein purification.T/F

Answer 3

True. Protein needs to be separated from the other material.

Question 4

If a protein of interest is an enzyme: then having an observable reaction product can be the basis of an assay. T/F

Answer 4

True. Enzymes are measured by their catalytic activity. Through light absorption of the product.

Question 5

If a protein of interest is an enzyme: then purification of the protein will destroy the enzyme’s activity.T/F

Answer 5

False. Purification is measured by the product.

Question 6

If a protein of interest is an enzyme: then it cannot be separated using the same methods used for other proteins. T/F

Answer 6

False.

Question 7

If the protein of interest is an enzyme: then there is no reason to purify it. T/F

Answer 7

False

Question 8

What are the fundamental principles of the separation of molecules using chromatography?

Answer 8

Discriminates using size (gel) or charge (electrophoresis). They are then positionally compared.

Question 9

What is the purpose of using x-ray crystallography?

Answer 9

To determine shape

Question 10

What is the purpose of using the improved method of Pehr Edman?

Answer 10

To label N terminals and identify them with spectroscopy.

Question 11

What is the purpose of using mass spectrometry?

Answer 11

To distinguish between what compounds are present.

Question 12

What are the purposes, characteristics and results of limited proteolysis?

Answer 12

Purpose: to separate and distinguish proteins
Characteristics: chemical agents or enzymes are used to split proteins.
Results: amino acids in proteins observed.

Question 13

List four important tools for proteomics.

Answer 13

Mass spectroscopy
Sequence databases.
Bioinformatics software
Separation techniques

Question 14

What are the big limitation to solving tertiary structures using the technique of two-dimensional NMR?

Answer 14

Not all isotopes have spin.

Question 15

What is the big limitation to solving tertiary structures using the technique of x-ray crystallogrophy?

Answer 15

Resolution