Proteomics Flashcards Preview

Block 2: Cardio, Renal, and Respiratory > Proteomics > Flashcards

Flashcards in Proteomics Deck (11):
1


How many genes in the human genome (known today)?


20,333

2


How many proteins in the cardiac proteome?

100,000 - 200,000

3


How many proteins in the plasma proteome?


5,000 - 20,000

4


How many pathways/networks in the cardiac proteome?


200 - 500

But we're only confident about 30 - 50 of them

5


How does the same human gene/genome make so many proteins?


Alternative splicing, but that's not the point here

Post-translational modifications (phosphorylation, acetylation, myristoylation, etc)

Location, abundance, and interactions with other proteins

6


What are the three forms of cell death in the heart?


Apoptosis

Autophagy

Necrosis

7


Why is it good to find biomarkers in the plasma rather than in the heart?


Because then you can predict disease, guide treatment, etc. without having to take a heart biopsy! Just need to take blood sample

8


What are two key technologies in proteomics?


1) Separating complex protein mixtures

2) Characterizing the proteins in the mixtures

9


What makes a good protein biomarker?


1) Easy to measure, reproducible

2) Provides incremental information

3) Helps guide clinical decision making

10


How do we best use biomarkers in medicine today?


Look at a panel of (3 or so) biomarkers to increase predictive value

Ex: Troponin I, CRP, BNP together predict risk of acute coronary syndromes

11


What exactly is a proteomics platform?


Technologies to analyze the proteome