Pyruvate Dehydrogenase Flashcards
What does pyruvate dehydrogenase complex (PDC) do
converts pyruvate into Acetyl CoA
Reactants of PDC
Pyruvate + NAD+ + CoASH
products of PDC
Acetyl CoA + CO2 + NADH + H+
3 enzymes in PDC
1) Pyruvate dehydrogenase
2) Dihydrolipoyl transacetylase
3) Dihydrolipoyl dehydrogenase
5 Cofactors in PDC
1) Thiamine pyrophosphate (TPP)
2) Lipoamide
3) NAD+
4) FAD
5) CoAsH
What does Thiamine pyrophosphate bind to
Pyruvate dehydrogenase (E1)
What does lipoamide bind to
dihydrolipoyl transacetylase (E2)
What does NAD+ bind to
nothing –> it’s free
What does CoASH bind to
nothing –> it’s free
what does FAD bind to
dihydrolipoyl dehydrogenase (E3)
What is Coenzyme A composed of
ADP, pentothenate (vit B5) and beta-mercaptoethylamine
how is lipoamide formed
lipoic acid is attached to a lysine R group in E2
what does lipoamide do
oxidized aldehydes to acyl groups, so acyl groups are bound to disulfide group
how does lipoamide act
like a ‘robotic arm’
what is the reduced form of lipoamide
dihydrolipoyl group
