qia idnaptex

Question 1

products needed in ESR1kit not provided

Answer 1

REAGENT AND EQUIPMENT
To use Primers and Probes set, the following is required
but not included:
- QIAcuity 5 Plex dPCR system
- QIAcuity Probe PCR kit
- QIAcuity Nanoplate 26K
- Optional: Internal Control of Extraction (ICE;
ref. 1171-000000-96)

Question 2

reaction setup

Answer 2

REACTION SETUP
Thaw all components at room temperature. Mix
thoroughly by vortexing each tube to ensure
homogeneity. Centrifuge briefly to collect content at
the bottom of each tube and store protected from
light.
1. Reconstitute the Amplification Reaction Mixture
(ARM) by adding 250 μL of QIAcuity Probe PCR
mastermix in one vial of PRIMEX. Mix thoroughly
by vortexing and centrifuge briefly to ensure that
all components are at the bottom of each tube.
Let sit at room temperature for about 3 minutes.
2. Prepare samples at the desired concentration
before setting up the reaction mixture according
to Table 2.
3. Process the TPC, as a sample. It should be used to
validate the test. Expected results are shown in
Figure 1.
4. Setup the PCR reaction as described in Table 2
and make sure that the ARM and the sample are
well homogenized.
5. Transfer the reaction mix from the reaction tubes
to the QIAcuity Nanoplate 26k wells.
6. Follow instructions for thermal cycling described
in Table 3.
Table 2: Setup of PCR reaction mix
Component Volume per reaction
Final
concentration
ARM 15 μL 1X
Sample 25 μL Up to 100 ng
Total Volume 40 μL -